Description: This FlowCytomix Simplex Kit is designed for the measurement of Human IL-9 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect IL-9 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
This kit contains bead population A10.
IL?9 is a proinflammatory cytokine historically believed to be involved in type 2 immune responses. However, recent evidence suggests IL?9 may be secreted by other T Helper lineages such as Treg and Th17 in addition to a new category called Th9. This Th9 lineage can either be derived from Th2 cells with TGF-beta or differentiated directly from na‹ve CD4+ T cells with TGF-beta and IL?4. The IL-9 expression in subsets such as Treg and Th17 illustrates the plasticity of cells to reprogram to alternative fates.
IL?9 is a member of the common cytokine receptor gamma chain?dependent family of cytokines which also includes IL?2, IL?4, IL?7, IL?15 and IL?21.
IL-9 is an extensively glycosylated protein of 14 kDa containing ten cysteine residues involved in disulfide bonding. The human gene maps to chromosome 5q31-32 which is a chromosomal region sometimes deleted in patients with myelodysplastic syndrome. Its pleiotropic effects on Th2 lymphocytes, B lymphocytes, mast cells, eosinophils, IgE production and gut and airway epithelial cells have implicated IL?9 in asthma and other allergy?related disorders. IL-9 is expressed by Reed-Sternberg cells and Hodgkin lymphoma cells and some large aplastic lymphoma cells, while non-Hodgkin lymphomas and peripheral T-cell lymphomas do not express it.
