Human IL-7 Instant ELISA

Description: The human IL-7 Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Interleukin-7. The human IL-7 Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin-7 is a pleiotropic cytokine with a variety of activities. It was first described as a growth factor expressed by stromal cells supporting the proliferation of precursor B-lymphocytes. IL-7 is furthermore produced by kidney and liver cells and keratinocytes exerting its bioactivities on spleen, thymus, B-, T-, NK-, and myeloid cells.

The gene for human IL-7 encodes for a protein comprising 152 amino acid residues. IL-7 interacts with its target cells via binding to specific cell surface receptors. There are three classes of IL-7 receptors described, exhibiting low, intermediate and high-binding affinity for IL-7.

Apart from B-cell proliferation IL-7 is involved in T-cell development in the thymus through thymocytes proliferation. IL-7 supports differentiation of cytotoxic T-lymphocytes and promotes the generation of lymphokine-activated killer-cells. IL-7 is further responsible for the upregulation of the production of pro-inflammatory cytokines and the stimulation of the tumoricidal activity of monocytes/macrophages.

Citations: Scholz C.,L St„rck,G Willimsky,T Blankenstein ,B D”rken and P T Daniel. Adenoviral transduction of tumor cells induces apoptosis in co-cultured T lymphocytes. Gene Therapy 2002;9:1438-1446. (Link)

References: Armitage,R.J.; Ziegler,S.F.; Friend,D.J.; Park,L.S.; Fanslow,W.C.. Identification of a novel low-affinity receptor for human interleukin-7. Blood 1992;79:1738-1745. (Link)

Namen,A.E.; Schmierer,A.E.; March,C.J.; Overell,R.W.; Park,L.S.; Urdal,D.L.; Mochizuki,D.Y.. B cell precursor growth-promoting activity. Purification and characterization of a growth factor active on lymphocyte precursors. J.Exp.Med. 1988;167:988-1002. (Link)

Heufler,C.; Topar,G.; Grasseger,A.; Stanzl,U.; Koch,F.; Romani,N.; Namen,A.E.; Schuler,G.. Interleukin 7 is produced by murine and human keratinocytes 50. J.Exp.Med. 1993;178:1109-1114. (Link)

Namen,A.E.; Lupton,S.; Hjerrild,K.; Wignall,J.; Mochizuki,D.Y.; Schmierer,A.; Mosley,B.; March,C.J.; Urdal,D.; Gillis,S.. Stimulation of B-cell progenitors by cloned murine interleukin-7. Nature 1988;333:571-573. (Link)