Human IL-6 High Sensitivity ELISA

Also known as: Interferon-beta 2, B-cell stimulatory factor 2,HGF

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS213HS*

Cat. No. Size
BMS213HS 96 tests
Please inquire about custom configurations or bulk packaging.

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Data for Human IL-6 High Sensitivity ELISA .

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  • Data for Human IL-6 High Sensitivity ELISA .
Description

Description: This High Sensitivity ELISAs enables the analysis of Interleukin 6 in low concentrations from serum, plasma, or cell culture samples.

eBioscience High Sensitivity ELISA Kits offer accurate quantification below conventional ELISA limits. This is made possible by ther inherent design by amplifying the signal generated by the enzyme horseradish peroxidase (HRP) and the colorimetric-detectable substrate tetramethylbenzidin (TMB) based on Tyramide Signal Amplification (TSA) technology*.

Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines,lipopolysaccharide (LPS) or viral infections. IL-6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells.

*High Sensitivity ELISAs are based on a patent from PerkinElmer LAS, patent number 5,731,158; 5,583,001; 5,196,306.

Details
Reactivity Human
Sample Volume 50 uL
Suitable Sample Types cell culture supernatant, serum, plasma (EDTA, heparin)
Sensitivity 0.03 pg/mL
Standard Curve Range 0.08 - 5.0 pg/mL
Components Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-6
Biotin-Conjugate anti-human IL-6 monoclonal antibody
Streptavidin-HRP
Human IL-6 Standard lyophilized, 200 pg/mL upon reconstitution
Control high, lyophilized
Control low, lyophilized
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Sample Diluent
Amplification Diluent Concentrate (2x)
Amplification Reagent I*
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films
* reagent contains ethyl alcohol
Reported Applications ELISA
Documentation
TDS Link Download TDS
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References

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