Human IL-5 Platinum ELISA

Description: The human IL-5 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-5. The human IL-5 ELISA is for in vitro diagnostic use. Not for use in therapeutic procedures.

Human IL-5 has been described as T-cell replacing factor and as B-cell growth factor II based on its induction of proliferation and Ig secretion by activated B cells. Several other activities have furthermore been attributed to this cytokine, including induction of growth and differentiation of eosinophils from bone marrow stem cells, induction of mIL-2R expression on activated B cells and enhancements of IgA secretion by LPS-stimulated B-cells. IL-5 has been shown to be produced by the TH2 subset of human TH clones. The spectrum of biological activities of IL-5, on B cells as well as other cell types suggests that IL-5 plays an important functional role in the activities of TH2 cells. IL-5 is a heavily glycosylated glycoprotein.

Citations: Paccani,Silvia Rossi; Tonello,Fiorella; Ghittoni,Raffaella; Natale,Mariarita; Muraro,Lucia; D'Elios,Mario Milco; Tang,Wei Jen; Montecucco,Cesare; Baldari,Cosima T.. Anthrax toxins suppress T lymphocyte activation by disrupting antigen receptor signaling. Journal of Experimental Medicine 2005;201:325-331. (Link)

Pacheco-Castro,Alberto; Alvarez-Zapata,David; Serrano-Torres,Pilar; Regueiro,Jose R.. Signaling Through a CD3{gamma}-Deficient TCR/CD3 Complex in Immortalized Mature CD4+ and CD8+ T Lymphocytes. The Journal of Immunology 1998;161:3152-3160. (Link)

References: Kinashi,T.; Harada,N.; Severinson,E.; Tanabe,T.; Sideras,P.; Konishi,M.; Azuma,C.; Tominaga,A.; Bergstedt-Lindqvist,S.; Takahashi,M.; .. Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II. Nature 1986;324:70-73. (Link)

Cherwinski,H.M.; Schumacher,J.H.; Brown,K.D.; Mosmann,T.R.. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J.Exp.Med. 1987;166:1229-1244. (Link)

Harada,N.; Matsumoto,M.; Koyama,N.; Shimizu,A.; Honjo,T.; Tominaga,A.; Takatsu,K.. T cell replacing factor/interleukin 5 induces not only B-cell growth and differentiation, but also increased expression of interleukin 2 receptor on activated B-cells. Immunol.Lett. 1987;15:205-215. (Link)

Coffman,R.L.; Shrader,B.; Carty,J.; Mosmann,T.R.; Bond,M.W.. A mouse T cell product that preferentially enhances IgA production. I. Biologic characterization. J.Immunol. 1987;139:3685-3690. (Link)

Sanderson,C.J.; O'Garra,A.; Warren,D.J.; Klaus,G.G.. Eosinophil differentiation factor also has B-cell growth factor activity: proposed name interleukin 4. Proc.Natl.Acad.Sci.U S A 1986;83:437-440. (Link)

Schumacher,J.H.; O'Garra,A.; Shrader,B.; van,Kimmenade A.; Bond,M.W.; Mosmann,T.R.; Coffman,R.L.. The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent. J.Immunol. 1988;141:1576-1581. (Link)

Loughnan,M.S.; Takatsu,K.; Harada,N.; Nossal,G.J.. T-cell-replacing factor (interleukin 5) induces expression of interleukin 2 receptors on murine splenic B cells. Proc.Natl.Acad.Sci.U.S.A 1987;84:5399-5403. (Link)

Azuma,C.; Tanabe,T.; Konishi,M.; Kinashi,T.; Noma,T.; Matsuda,F.; Yaoita,Y.; Takatsu,K.; Hammarstrom,L.; Smith,C.I.; .. Cloning of cDNA for human T-cell replacing factor (interleukin-5) and comparison with the murine homologue. Nucleic Acids Res. 1986;14:9149-9158. (Link)

Takatsu,K.; Tominaga,A.; Hamaoka,T.. Antigen-induced T cell-replacing factor (TRF). I. Functional characterization of a TRF-producing helper T cell subset and genetic studies on TRF production. J.Immunol. 1980;124:2414-2422. (Link)