Human IL-4 High Sensitivity ELISA

Also known as: B-cell stimulatory factor 1, B cell growth factor 1

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# BMS225HS*

Cat. No. Size
BMS225HS 96 tests
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Data for Human IL-4 High Sensitivity ELISA .

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  • Data for Human IL-4 High Sensitivity ELISA .
Description

Description: This High Sensitivity ELISAs enables the analysis of Interleukin- 4 in low concentrations from serum, plasma, or cell culture samples.
eBioscience High Sensitivity ELISA Kits offer accurate quantification below conventional ELISA limits. This is made possible by ther inherent design by amplifying the signal generated by the enzyme horseradish peroxidase (HRP) and the colorimetric-detectable substrate tetramethylbenzidin (TMB) based on Tyramide Signal Amplification (TSA) technology*.

Human IL-4 exists in molecular weight forms between 15,000 and 19,000 daltons, representing variable glycosylation. The gene for human IL-4 is found on the long arm of chromsome 5 in close association with genes for IL-13, IL-5 GM-CSF and IL-3. IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M. Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be up-regulated by IL-2 and IL-4.
IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.

*High Sensitivity ELISAs are based on a patent from PerkinElmer LAS, patent number 5,731,158; 5,583,001; 5,196,306.

Details
Reactivity Human
Sample Volume 50 uL
Suitable Sample Types cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sensitivity 0.1 pg/mL
Standard Curve Range 0.25 - 16.0 pg/mL
Components Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-4
Biotin-Conjugate anti-human IL-4 polyclonal antibody
Streptavidin-HRP
Human IL-4 Standard lyophilized, 1 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Amplification Diluent Concentrate (2x)
Amplification Reagent I*
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution
Stop Solution (1M Phosphoric acid)
Adhesive Films
* reagent contains ethyl alcohol
Reported Applications ELISA
Documentation
TDS Link Download TDS
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References

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Castellano-Higuera,Ana; Gonzalez-Reimers,Emilio; Aleman-Valls,M.Remedios; Abreu-Gonzalez,Pedro; Santolaria-Fernandez,Francisco; Vega-Prieto,Maria Jose De La; Gomez-Sirvent,Juan Luis; Pelazas-Gonzalez,Ricardo. Cytokines and lipid peroxidation in alcoholics with chronic hepatitis C virus infection. Alcohol and Alcoholism 2008;43:137-142. (Link)

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