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Human IL-4 High Sensitivity ELISA

Also known as: B-cell stimulatory factor 1, B cell growth factor 1

RUO: For Research Use Only. Not for use in diagnostic procedures.

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Cat. No. Size
BMS225HS 96 tests

Data for Human IL-4 High Sensitivity ELISA .

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  • Data for Human IL-4 High Sensitivity ELISA .

Description: This High Sensitivity ELISAs enables the analysis of Interleukin- 4 in low concentrations from serum, plasma, or cell culture samples.
eBioscience High Sensitivity ELISA Kits offer accurate quantification below conventional ELISA limits. This is made possible by ther inherent design by amplifying the signal generated by the enzyme horseradish peroxidase (HRP) and the colorimetric-detectable substrate tetramethylbenzidin (TMB) based on Tyramide Signal Amplification (TSA) technology*.

Human IL-4 exists in molecular weight forms between 15,000 and 19,000 daltons, representing variable glycosylation. The gene for human IL-4 is found on the long arm of chromsome 5 in close association with genes for IL-13, IL-5 GM-CSF and IL-3. IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M. Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be up-regulated by IL-2 and IL-4.
IL-4 is produced by a particular subset of T helper cells, the TH2 cells. These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.

*High Sensitivity ELISAs are based on a patent from PerkinElmer LAS, patent number 5,731,158; 5,583,001; 5,196,306.

Reactivity Human
Sample Volume 50 uL
Suitable Sample Types cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sensitivity 0.1 pg/mL
Standard Curve Range 0.25 - 16.0 pg/mL
Components Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-4
Biotin-Conjugate anti-human IL-4 polyclonal antibody
Human IL-4 Standard lyophilized, 1 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Amplification Diluent Concentrate (2x)
Amplification Reagent I*
Amplification Reagent II
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution
Stop Solution (1M Phosphoric acid)
Adhesive Films
* reagent contains ethyl alcohol
Reported Applications ELISA
TDS Link Download TDS
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Hamaguchi,Y.; Kanakura,Y.; Fujita,J.; Takeda,S.; Nakano,T.; Tarui,S.; Honjo,T.; Kitamura,Y.. Interleukin 4 as an essential factor for in vitro clonal growth of murine connective tissue-type mast cells. J.Exp.Med. 1987;165:268-273. (Link)

Lee,J.D.; Swisher,S.G.; Minehart,E.H.; McBride,W.H.; Economou,J.S.. Interleukin-4 downregulates interleukin-6 production in human peripheral blood mononuclear cells. J.Leukoc.Biol. 1990;47:475-479. (Link)

Plaut,M.; Pierce,J.H.; Watson,C.J.; Hanley-Hyde,J.; Nordan,R.P.; Paul,W.E.. Mast cell lines produce lymphokines in response to cross-linkage of Fc epsilon RI or to calcium ionophores. Nature 1989;339:64-67. (Link)

Puri,R.K.; Finbloom,D.S.; Leland,P.; Mostowski,H.; Siegel,J.P.. Expression of high-affinity IL-4 receptors on murine tumour infiltrating lymphocytes and their up-regulation by IL-2. Immunology 1990;70:492-497. (Link)

Sideras,P.; Bergstedt-Lindqvist,S.; MacDonald,H.R.; Severinson,E.. Secretion of IgG1 induction factor by T cell clones and hybridomas. Eur.J.Immunol. 1985;15:586-593. (Link)

Lowenthal,J.W.; Castle,B.E.; Christiansen,J.; Schreurs,J.; Rennick,D.; Arai,N.; Hoy,P.; Takebe,Y.; Howard,M.. Expression of high affinity receptors for murine interleukin 4 (BSF-1) on hemopoietic and nonhemopoietic cells. J.Immunol. 1988;140:456-464. (Link)

Park,L.S.; Friend,D.; Grabstein,K.; Urdal,D.L.. Characterization of the high-affinity cell-surface receptor for murine B-cell-stimulating factor 1. Proc.Natl.Acad.Sci.U.S.A 1987;84:1669-1673. (Link)

Brown,M.; Hu-Li,J.; Paul,W.E.. IL-4/B cell stimulatory factor 1 stimulates T cell growth by an IL-2-independent mechanism. J.Immunol. 1988;141:504-511. (Link)

Foxwell,B.M.; Woerly,G.; Ryffel,B.. Identification of interleukin 4 receptor-associated proteins and expression of both high- and low-affinity binding on human lymphoid cells. Eur.J.Immunol. 1989;19:1637-1641. (Link)

Spits,H.; Yssel,H.; Paliard,X.; Kastelein,R.; Figdor,C.; de Vries,J.E.. IL-4 inhibits IL-2-mediated induction of human lymphokine-activated killer cells, but not the generation of antigen-specific cytotoxic T lymphocytes in mixed leukocyte cultures. J.Immunol. 1988;141:29-36. (Link)

Toi,M.; Harris,A.L.; Bicknell,R.. Interleukin-4 is a potent mitogen for capillary endothelium. Biochem.Biophys.Res.Commun. 1991;174:1287-1293. (Link)

Hart,P.H.; Vitti,G.F.; Burgess,D.R.; Whitty,G.A.; Piccoli,D.S.; Hamilton,J.A.. Potential antiinflammatory effects of interleukin 4: suppression of human monocyte tumor necrosis factor alpha, interleukin 1, and prostaglandin E2. Proc.Natl.Acad.Sci.U S A 1989;86:3803-3807. (Link)

Coffman,R.L.; Ohara,J.; Bond,M.W.; Carty,J.; Zlotnik,A.; Paul,W.E.. B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells. J.Immunol. 1986;136:4538-4541. (Link)

Grabstein,K.H.; Park,L.S.; Morrissey,P.J.; Sassenfeld,H.; Price,V.; Urdal,D.L.; Widmer,M.B.. Regulation of murine T cell proliferation by B cell stimulatory factor-1. J.Immunol. 1987;139:1148-1153. (Link)

Roehm,N.W.; Leibson,H.J.; Zlotnik,A.; Kappler,J.; Marrack,P.; Cambier,J.C.. Interleukin-induced increase in Ia expression by normal mouse B cells. J.Exp.Med. 1984;160:679-694. (Link)

Mosmann,T.R.; Bond,M.W.; Coffman,R.L.; Ohara,J.; Paul,W.E.. T-cell and mast cell lines respond to B-cell stimulatory factor 1. Proc.Natl.Acad.Sci.U.S.A 1986;83:5654-5658. (Link)