Human IL-23 FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human IL-23 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect IL-23 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

NEW BEADSET: Now combinable with IL-12p70 (BMS8238FF*)

This kit contains bead population B3.

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked to p19. Like p35 of IL-12, p19 is biologically inactive by itself. IL-23 interacts with IL-12R beta -1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-gamma production by na‹ve and memory T cells, as compared to IL-12.

eBioscience's recombinant IL-23 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p19 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.

References: Layh-Schmitt,G.; Colbert,R.A.. The interleukin-23/interleukin-17 axis in spondyloarthritis. Curr.Opin.Rheumatol. 2008;20:392-397. (Link)

Lee,E.; Trepicchio,W.L.; Oestreicher,J.L.; Pittman,D.; Wang,F.; Chamian,F.; Dhodapkar,M.; Krueger,J.G.. Increased expression of interleukin 23 p19 and p40 in lesional skin of patients with psoriasis vulgaris. J.Exp.Med. 2004;199:125-130. (Link)

Cua,D.J.; Sherlock,J.; Chen,Y.; Murphy,C.A.; Joyce,B.; Seymour,B.; Lucian,L.; To,W.; Kwan,S.; Churakova,T.; Zurawski,S.; Wiekowski,M.; Lira,S.A.; Gorman,D.; Kastelein,R.A.; Sedgwick,J.D.. Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain. Nature 2003;421:744-748. (Link)

Lankford,C.S.; Frucht,D.M.. A unique role for IL-23 in promoting cellular immunity. J.Leukoc.Biol. 2003;73:49-56. (Link)

Maloy,K.J.. The Interleukin-23 / Interleukin-17 axis in intestinal inflammation. J Intern.Med 2008;263:584-590. (Link)

Holscher,C.. The power of combinatorial immunology: IL-12 and IL-12-related dimeric cytokines in infectious diseases. Med Microbiol.Immunol 2004;193:1-17. (Link)

Tan,Z.Y.; Bealgey,K.W.; Fang,Y.; Gong,Y.M.; Bao,S.. Interleukin-23: Immunological roles and clinical implications. Int J Biochem.Cell Biol 2008;(Link)

Lankford,C.S.; Frucht,D.M.. A unique role for IL-23 in promoting cellular immunity. J Leukoc.Biol 2003;73:49-56. (Link)

Cordoba-Rodriguez,R.; Frucht,D.M.. IL-23 and IL-27: new members of the growing family of IL-12-related cytokines with important implications for therapeutics. Expert.Opin.Biol.Ther. 2003;3:715-723. (Link)

Brombacher,F.; Kastelein,R.A.; Alber,G.. Novel IL-12 family members shed light on the orchestration of Th1 responses. Trends Immunol. 2003;24:207-212. (Link)

Frucht,D.M.. IL-23: a cytokine that acts on memory T cells. Sci.STKE. 2002;2002:E1(Link)

Goriely,S.; Goldman,M.. Interleukin-12 family members and the balance between rejection and tolerance. Curr.Opin.Organ Transplant 2008;13:4-9. (Link)

Vandenbroeck,K.; Alloza,I.; Gadina,M.; Matthys,P.. Inhibiting cytokines of the interleukin-12 family: recent advances and novel challenges. J Pharm.Pharmacol 2004;56:145-160. (Link)

Aggarwal,S.; Ghilardi,N.; Xie,M.H.; de Sauvage,F.J.; Gurney,A.L.. Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17. J Biol Chem. 2003;278:1910-1914. (Link)

Brombacher,F.; Kastelein,R.A.; Alber,G.. Novel IL-12 family members shed light on the orchestration of Th1 responses. Trends Immunol 2003;24:207-212. (Link)

Vandenbroeck,K.; Alloza,I.; Gadina,M.; Matthys,P.. Inhibiting cytokines of the interleukin-12 family: recent advances and novel challenges. J Pharm.Pharmacol 2004;56:145-160. (Link)

Brombacher,F.; Kastelein,R.A.; Alber,G.. Novel IL-12 family members shed light on the orchestration of Th1 responses. Trends Immunol. 2003;24:207-212. (Link)

Lee,E.; Trepicchio,W.L.; Oestreicher,J.L.; Pittman,D.; Wang,F.; Chamian,F.; Dhodapkar,M.; Krueger,J.G.. Increased expression of interleukin 23 p19 and p40 in lesional skin of patients with psoriasis vulgaris. J Exp Med 2004;199:125-130. (Link)

McGeachy,M.J.; Cua,D.J.. Th17 cell differentiation: the long and winding road. Immunity 2008;28:445-453. (Link)