Human IL-2 High Sensitivity ELISA

Description: This High Sensitivity ELISAs enables the analysis of Interleukin-2 in low concentrations from serum, plasma, or cell culture samples.

eBioscience High Sensitivity ELISA Kits offer accurate quantification below conventional ELISA limits. This is made possible by ther inherent design by amplifying the signal generated by the enzyme horseradish peroxidase (HRP) and the colorimetric-detectable substrate tetramethylbenzidin (TMB) based on Tyramide Signal Amplification (TSA) technology*.

IL-2 plays a central role in the activation and proliferation of lymphocytes that have been primed by antigens. IL-2 plays a pivotal role in for the expansion of most T-cells, natural killer cells and B-cells during certain phases of their response. IL-2 is a 15 kDa glycoprotein encoded by a single gene located in the q26-28 region of human chromosome 4. The cDNA deduced polypeptide consists of 153 amino acids. Apart from its most important role to mediate antigen-specific T-lymphocyte proliferation, IL-2 modulates the expression of interferon gamma and major histocompatibility antigens, stimulates proliferation and differentiation of activated B-cells, augments natural killer cell activity and inhibits granulocyte-macrophage colony formation. IL-2 displays antitumoral effects, thus being used in cancer therapy. Monitoring of IL-2 levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes.

*High Sensitivity ELISAs are based on a patent from PerkinElmer LAS, patent number 5,731,158; 5,583,001; 5,196,306.

Citations: Paccani,Silvia Rossi; Tonello,Fiorella; Ghittoni,Raffaella; Natale,Mariarita; Muraro,Lucia; D'Elios,Mario Milco; Tang,Wei Jen; Montecucco,Cesare; Baldari,Cosima T.. Anthrax toxins suppress T lymphocyte activation by disrupting antigen receptor signaling. Journal of Experimental Medicine 2005;201:325-331. (Link)

Falk,Markus; Ussat,Sandra; Reiling,Norbert; Wesch,Daniela; Kabelitz,Dieter; Adam-Klages,Sabine. Caspase Inhibition Blocks Human T Cell Proliferation by Suppressing Appropriate Regulation of IL-2, CD25, and Cell Cycle-Associated Proteins. The Journal of Immunology 2004;173:5077-5085. (Link)

Gunaydin,Serdar; Ayrancioglu,Kamil; Dikmen,Erkan; McCusker,Kevin; Vijay,Venkataramana; Sari,Tamer; Tezcaner,Tevfik; Zorlutuna,Yaman. Clinical effects of leukofiltration and surface modification on post-cardiopulmonary bypass atrial fibrillation in different risk cohorts. Perfusion 2007;22:279-288. (Link)

Lacomba,Manuel Santos; Martin,Carmen Marcos; Chamond,Rafael Ramirez; Galera,Jose Maria Gallardo; Omar,Mohamed; Estevez,Eduardo Collantes. Aqueous and Serum Interferon {gamma}, Interleukin (IL) 2, IL-4, and IL-10 in Patients With Uveitis. Archives of Ophthalmology 2000;118:768-772. (Link)

ALLENDE; CORELL; MANZANARES; MADRUGA; MARCOS; MADRONO; LOPEZ,G.O.Y.A.; GARCIA,P.E.R.E.; MORENO; RODRIGO; SANZ; ARNAIZ,V.I.L.L.. Immunodeficiency associated with anorexia nervosa is secondary and improves after refeeding. Immunology 1998;94:543-551. (Link)

Gunaydin,Serdar; McCusker,Kevin; Vijay,Venkataramana; Isbir,Selim; Sari,Tamer; Onur,Mehmet Ali; Gurpinar,Aylin; Sezgin,Aysun; Sargon,Mustafa F.; Tezcaner,Tevfik; Zorlutuna,Yaman. Comparison of polymethoxyethylacrylate-coated circuits with leukocyte filtration and reduced heparinization protocol on heparin-bonded circuits in different risk cohorts. Perfusion 2006;21:329-342. (Link)

Gobert,Michael; Treilleux,Isabelle; Bendriss-Vermare,Nathalie; Bachelot,Thomas; Goddard-Leon,Sophie; Arfi,Vanessa; Biota,Cathy; Doffin,Anne Claire; Durand,Isabelle; Olive,Daniel; Perez,Solene; Pasqual,Nicolas; Faure,Christelle; Ray-Coquard,Isabelle; Puisieux,Alain; Caux,Christophe; Blay,Jean Yves; Menetrier-Caux,Christine. Regulatory T Cells Recruited through CCL22/CCR4 Are Selectively Activated in Lymphoid Infiltrates Surrounding Primary Breast Tumors and Lead to an Adverse Clinical Outcome. Cancer Research 2009;69:2000-2009. (Link)

Pacheco-Castro,Alberto; Alvarez-Zapata,David; Serrano-Torres,Pilar; Regueiro,Jose R.. Signaling Through a CD3{gamma}-Deficient TCR/CD3 Complex in Immortalized Mature CD4+ and CD8+ T Lymphocytes. The Journal of Immunology 1998;161:3152-3160. (Link)

References: Naldini,A.; Fleischmann,W.R.,Jr.; Ballas,Z.K.; Klimpel,K.D.; Klimpel,G.R.. Interleukin 2 inhibits in vitro granulocyte-macrophage colony formation. J.Immunol. 1987;139:1880-1884. (Link)

Tigges,M.A.; Casey,L.S.; Koshland,M.E.. Mechanism of interleukin-2 signaling: mediation of different outcomes by a single receptor and transduction pathway. Science 1989;243:781-786. (Link)

Gressler,V.H.; Weinkauff,R.E.; Franklin,W.A.; Golomb,H.M.. Modulation of the expression of major histocompatibility antigens on splenic hairy cells--differential effect upon in vitro treatment with alpha-2b-interferon, gamma-interferon, and interleukin-2. Blood 1988;72:1048-1053. (Link)

Phadke,K.; Carlson,D.G.; Gitter,B.D.; Butler,L.D.. Role of interleukin 1 and interleukin 2 in rat and mouse arthritis models. J.Immunol. 1986;136:4085-4091. (Link)

Cornaby,A.; Simpson,M.; Vann,Rice R.; Dempsey,R.; Madras,P.; Jenkins,R.; Monaco,A.. Interleukin 2 levels and urine cytology distinguish between cyclosporine toxicity and rejection in renal and liver allograft recipients. Transplant Proc. 1988;20:827-830. (Link)