Human IL-18 Platinum ELISA

Description: The human IL-18 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-18. The human IL-18 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Formerly called interferon gamma inducing factor (IGIF), IL-18 is a cytokine that plays an important role in the Th1 response, primarily by its ability to induce IFN gamma production in T cells and natural killer cells.
In terms of structure, IL-18 and IL-1 beta share primary amino acid sequences of the so-called "signature sequence" motif and are similarly folded as all beta pleated sheet molecules. Also similar to IL-1 beta, IL- 18 is synthesized as a biologically inactive precursor molecule lacking a signal peptide which requires cleavage into an active, mature molecule by the intracellular cysteine protease called IL-1 beta-converting enzyme (ICE, caspase-1). The activity of the mature IL-18 is closely related to that of IL-1.
The activity of IL-18 is via an IL-18 receptor complex. IL-18 induces gene expression and synthesis of TNF, IL-1, Fas Ligand, and several chemokines. Similar to IL-1, IL-18 participates in both innate and acquired immunity.

Citations: Sun,Yong Jiang; Lim,T.K.; Ong,Adrian Kheng Yeow; Ho,Benjamin Choon Heng; Seah,Geok Teng; Paton,Nicholas I.. Tuberculosis associated with Mycobacterium tuberculosis Beijing and non-Beijing genotypes: a clinical and immunological comparison. BMC Infectious Diseases 2006;6:105(Link)

Kolb-Maurer,Annette; Kammerer,Ulrike; Maurer,Mathias; Gentschev,Ivaylo; Brocker,Eva Bettina; Rieckmann,Peter; Kampgen,Eckhart. Production of IL-12 and IL-18 in human dendritic cells upon infection by Listeria monocytogenes. FEMS Immunology and Medical Microbiology 2003;35:255-262. (Link)

Xu,Guangwu; Nie,Hong; Li,Ningli; Zheng,Wenxin; Zhang,Dongqing; Feng,Guozhang; Ni,Liqing; Xu,Rong; Hong,Jian; Zhang,Jingwu Z.. Role of osteopontin in amplification and perpetuation of rheumatoid synovitis. Journal of Clinical Investigation 2005;115:1060-1067. (Link)

Fiorina,Paolo; Folli,Franco; Ferrero,Elisabetta; Orsenigo,Elena; Finzi,Giovanna; Mazzolari,Gabriella; Placidi,Claudia; Perego,Lucia; Rosa,Stefano La; Melandri,Marco; Monti,Lucilla; Capella,Carlo; D'Angelo,Armando; Staudacher,Carlo; Secchi,Antonio. Morphological and functional differences in haemostatic axis between kidney transplanted and end-stage renal disease patients. Transplant International 2005;18:1036-1047. (Link)

Chen,D.Y.; Lan,J.L.; Lin,F.J.; Hsieh,T.Y.; Wen,M.C.. Predominance of Th1 cytokine in peripheral blood and pathological tissues of patients with active untreated adult onset Still's disease. Annals of the Rheumatic Diseases 2004;63:1300-1306. (Link)

Maczynska,Iwona; Millo,Barbara; Ratajczak-StefaDska,Violetta; Maleszka,Romuald; Szych,Zbigniew; Kurpisz,Maciej; Giedrys-Kalemba,Stefania. Proinflammatory cytokine (IL-1beta, IL-6, IL-12, IL-18 and TNF-alpha) levels in sera of patients with subacute cutaneous lupus erythematosus (SCLE). Immunology letters 2006;102:79-82. (Link)

Iannello,Alexandre; Samarani,Suzanne; Debbeche,Olfa; Ahmad,Rasheed; Boulassel,Mohamed Rachid; Tremblay,Cecile; Toma,Emil; Routy,Jean Pierre; Ahmad,Ali. Potential role of IL-18 in the immunopathogenesis of AIDS: Involvement in fratricidal killing of NK cells. Journal of Virology 2009;JVI(Link)

Strowig,Till; Brilot,Fabienne; Arrey,Frida; Bougras,Gwenola; Thomas,Dolca; Muller,William A.; Mnz,Christian. Tonsilar NK Cells Restrict B Cell Transformation by the Epstein-Barr Virus via IFN-Ý. PLoS Pathogens 2008;4:e27(Link)

Mohty,Mohamad; Vialle-Castellano,Alexandra; Nunes,Jacques A.; Isnardon,Daniel; Olive,Daniel; Gaugler,Beatrice. IFN-{alpha} Skews Monocyte Differentiation into Toll-Like Receptor 7-Expressing Dendritic Cells with Potent Functional Activities. The Journal of Immunology 2003;171:3385-3393. (Link)

Wang,H.H.; Lin,C.Y.. Interleukin-12 and -18 Levels in Peritoneal Dialysate Effluent Correlate With the Outcome of Peritonitis in Patients... American Journal of Kidney Diseases 2005;46:328-338. (Link)

Orozco,A.; Gemmell,E.; Bickel,M.; Seymour,G.J.. Interleukin-1 beta, interleukin-12 and interleukin-18 levels in gingival fluid and serum of patients with gingivitis and periodontitis. Oral Microbiology and Immunology 2006;21:256-260. (Link)

Panafidina,T.A.; Popkova,T.V.; Alekberova,Z.S.; Mach,E.S.; Novikova,D.S.; Novikov,A.A.; Alexandrova,E.N.; Nasonov,E.L.. INFLAMMATORY MARCERS IN WOMEN WITH SYSTEMIC LUPUS ERYTHEMATOSUS. EULAR Meeting Abstracts 2006;65:355(Link)

Wenwei,Liu; Qizhu,Tang; Hua,Jiang; Xiangwu,Ding; Yongsheng,Liu; Rui,Zhu; Yongqian,Tang; Bin,Li; Min,Wei. Promoter Polymorphism of Interleukin-18 in Angiographically Proven Coronary Artery Disease. Angiology 2009;60:180-185. (Link)

Kaser,Arthur; Kaser,Susanne; Kaneider,Nicole C.; Enrich,Barbara; Wiedermann,Christian J.; Tilg,Herbert. Interleukin-18 attracts plasmacytoid dendritic cells (DC2) and promotes Th1 induction by DC2 through IL-18 receptor expression. Blood 2003;2002-2007. (Link)

Schernthaner,G.H.; Plank,C.; Minar,E.; Bieglmayer,C.; Koppensteiner,R.; Schernthaner,G.. No effect of homocysteine-lowering therapy on vascular inflammation and haemostasis in peripheral arterial occlusive disease. European Journal of Clinical Investigation 2006;36:333-339. (Link)

References: Takubo,T.; Ohkura,H.; Kumura,T.; Ohta,K.; Yamane,T.; Hino,M.; Kamitani,T.; Tatsumi,N.. Serum IL-18 levels in patients with various hematological disorders. Haematologia (Budap.) 2000;30:31-33. (Link)

Nakamura,H.; Komatsu,K.; Ayaki,M.; Kawamoto,S.; Murakami,M.; Uoshima,N.; Yagi,T.; Hasegawa,T.; Yasumi,M.; Karasuno,T.; Teshima,H.; Hiraoka,A.; Masaoka,T.. Serum levels of soluble IL-2 receptor, IL-12, IL-18, and IFN-gamma in patients with acute graft-versus-host disease after allogeneic bone marrow transplantation. J.Allergy Clin.Immunol. 2000;106:S45-S50. (Link)

Companjen,A.R.; van,der Velden,V; Vooys,A.; Debets,R.; Benner,R.; Prens,E.P.. Human keratinocytes are major producers of IL-18: predominant expression of the unprocessed form 31. Eur.Cytokine Netw. 2000;11:383-390. (Link)

Dinarello,C.A.; Novick,D.; Puren,A.J.; Fantuzzi,G.; Shapiro,L.; Muhl,H.; Yoon,D.Y.; Reznikov,L.L.; Kim,S.H.; Rubinstein,M.. Overview of interleukin-18: more than an interferon-gamma inducing factor. J.Leukoc.Biol. 1998;63:658-664. (Link)

Golab,J.. Interleukin 18--interferon gamma inducing factor--a novel player in tumour immunotherapy? 46. Cytokine 2000;12:332-338. (Link)

Torre,D.; Speranza,F.; Martegani,R.; Pugliese,A.; Castelli,F.; Basilico,C.; Biondi,G.. Circulating levels of IL-18 in adult and paediatric patients with HIV-1 infection. AIDS 2000;14:2211-2212. (Link)

Tsutsui,H.; Matsui,K.; Okamura,H.; Nakanishi,K.. Pathophysiological roles of interleukin-18 in inflammatory liver diseases. Immunol.Rev. 2000;174:192-209. (Link)

Lebel-Binay,S.; Berger,A.; Zinzindohoue,F.; Cugnenc,P.; Thiounn,N.; Fridman,W.H.; Pages,F.. Interleukin-18: biological properties and clinical implications. Eur.Cytokine Netw. 2000;11:15-26. (Link)

Fantuzzi,G.; Dinarello,C.A.. Interleukin-18 and interleukin-1 beta: two cytokine substrates for ICE (caspase-1). J.Clin.Immunol. 1999;19:1-11. (Link)

Gillespie,M.T.; Horwood,N.J.. Interleukin-18: perspectives on the newest interleukin. Cytokine Growth Factor Rev. 1998;9:109-116. (Link)

Xu,D.; Trajkovic,V.; Hunter,D.; Leung,B.P.; Schulz,K.; Gracie,J.A.; McInnes,I.B.; Liew,F.Y.. IL-18 induces the differentiation of Th1 or Th2 cells depending upon cytokine milieu and genetic background. Eur.J.Immunol. 2000;30:3147-3156. (Link)

Ida,A.; Tsuji,Y.; Muranaka,J.; Kanazawa,R.; Nakata,Y.; Adachi,S.; Okamura,H.; Koyama,K.. IL-18 in pregnancy; the elevation of IL-18 in maternal peripheral blood during labour and complicated pregnancies.. J.Reprod.Immunol. 2000;47:65-74. (Link)

Grobmyer,S.R.; Lin,E.; Lowry,S.F.; Rivadeneira,D.E.; Potter,S.; Barie,P.S.; Nathan,C.F.. Elevation of IL-18 in human sepsis. J.Clin.Immunol. 2000;20:212-215. (Link)

Gracie,J.A.; Forsey,R.J.; Chan,W.L.; Gilmour,A.; Leung,B.P.; Greer,M.R.; Kennedy,K.; Carter,R.; Wei,X.Q.; Xu,D.; Field,M.; Foulis,A.; Liew,F.Y.; McInnes,I.B.. A proinflammatory role for IL-18 in rheumatoid arthritis. J.Clin.Invest 1999;104:1393-1401. (Link)

Takubo,T.; Okura,H.; Kumura,T.; Nishiki,S.; Kinoshita,Y.; Koh,K.; Ohta,K.; Yamane,T.; Hino,M.; Kamitani,T.; Tatsumi,N.. Human IL-18 bioactivity in hematological malignancies with highly elevated serum IL-18 levels. Acta Haematol. 2000;103:162-164. (Link)

Pizarro,T.T.; Michie,M.H.; Bentz,M.; Woraratanadharm,J.; Smith,M.F.,Jr.; Foley,E.; Moskaluk,C.A.; Bickston,S.J.; Cominelli,F.. IL-18, a novel immunoregulatory cytokine, is up-regulated in Crohn's disease: expression and localization in intestinal mucosal cells. J.Immunol. 1999;162:6829-6835. (Link)

Monteleone,G.; Trapasso,F.; Parrello,T.; Biancone,L.; Stella,A.; Iuliano,R.; Luzza,F.; Fusco,A.; Pallone,F.. Bioactive IL-18 expression is up-regulated in Crohn's disease. J.Immunol. 1999;163:143-147. (Link)

Endo,S.; Inada,K.; Yamada,Y.; Wakabayashi,G.; Ishikura,H.; Tanaka,T.; Sato,S.. Interleukin 18 (IL-18) levels in patients with sepsis 37. J.Med. 2000;31:15-20. (Link)