Human IL-17A (homodimer) ELISA Ready-SET-Go!®

Also known as: Interleukin-17A, IL-17AA

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7176

Cat. No. Size
88-7176-22 2 x 96 tests w/plates
88-7176-76 20 x 96 tests w/plates
88-7176-77 20 x 96 tests
88-7176-86 10 x 96 tests w/plates
88-7176-88 10 x 96 tests
Please inquire about custom configurations or bulk packaging.

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Data for Human IL-17A (homodimer) ELISA Ready-SET-Go!®.

Standard curve of Human IL-17A (homodimer) ELISA Ready-SET-Go!® ...View More

  • Data for Human IL-17A (homodimer) ELISA Ready-SET-Go!®.
Description

Description: This Human IL-17A ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of human IL-17A homodimer protein levels from samples including serum, and supernatants from cell cultures. Minimal crossreactivity to the recombinant human Il-17AF heterodimer is observed at 0.4% when spiked in excess (100ng/ml).

Details
Reactivity Human
Sensitivity 4 pg/mL
Standard Curve Range 4 - 500 pg/mL
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation
TDS Link Download TDS
Related Products
Cat. No. Name Excite Emit Application Reg.
14-8178 Human IL-17AF Recombinant Protein ELISA, FA RUO
14-8179 Human IL-17A Recombinant Protein FA RUO
14-8479 Human IL-17F Recombinant Protein FA RUO
References

References: Gu Y, Hu X, Liu C, Qv X, Xu C. Interleukin (IL)-17 promotes macrophages to produce IL-8, IL-6 and tumour necrosis factor-alpha in aplastic anaemia. Br J Haematol. 2008 Jul;142(1):109-14. (RSG ELISA kit, BM and PB plasma, PubMed)

Hirota K, Yoshitomi H, Hashimoto M, Maeda S, Teradaira S, Sugimoto N, Yamaguchi T, Nomura T, Ito H, Nakamura T, Sakaguchi N, Sakaguchi S. Preferential recruitment of CCR6-expressing Th17 cells to inflamed joints via CCL20 in rheumatoid arthritis and its animal model. J Exp Med. 2007 Nov 26;204(12):2803-12. (RSG ELISA kit, synovial fluid, PubMed)

Saruta M, Yu QT, Avanesyan A, Fleshner PR, Targan SR, Papadakis KA. Phenotype and effector function of CC chemokine receptor 9-expressing lymphocytes in small intestinal Crohn's disease. J Immunol. 2007 Mar 1;178(5):3293-300. (RSG ELISA kit, TC supernatant, PubMed)