Human IL-13 FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human IL-13 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect IL-13 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population B4.

Human IL-13 is a nonglycosylated protein of 132 amino acids with a molecular weight of 12 kDa. The IL-13 gene is located on chromosome 5q23-31, in the same region as the genes encoding IL-3, IL-4, IL-5 and GM-CSF. It is produced by activated CD4+ and CD8+ T cells, but the expression seems to be more abundant in CD4+ T cells. Although it is a Th2 cytokine in the mouse, it appears to be produced by Th0, Th1, and Th2-like human T cell clones.
IL-13 induces CD23 expression on B cells, promotes B cell proliferation in combination with anti-Ig or CD40 antibodies, and stimulates secretion IgE, and IgG4. IL-13 has also been shown to prolong survival of human monocytes and increases surface expression of MHC class II, CD23, and members of the integrin superfamily, like CD11b, CD11c, CD18, CD29 and CD49e. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells.
The capacity of IL-13 to induce IgE synthesis indicates that IL-13 may play an important regulatory role in IgE-mediated atopic diseases. The measurement of IL-13 in body fluids may thus provide further information about the pathophysiology of atopic diseases.

Furthermore, IL-13 inhibits HIV-1 replication in primary culture-derived macrophages and represents a candidate cytokine for the suppression of HIV infection within monocytes and macrophages in vivo.

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