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Human IL-12 p70 ELISA Ready-SET-Go!®

Also known as: Interleukin-12, IL12, IL23

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7126

Cat. No. Size
88-7126-22 2 x 96 tests
88-7126-88 10 x 96 tests
88-7126-86 10 x 96 tests w/plates
88-7126-77 20 x 96 tests
88-7126-76 20 x 96 tests w/plates
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Data for Human IL-12 p70 ELISA Ready-SET-Go!®.

Standard curve of Human IL-12 p70 ELISA Ready-SET-Go!® ...View More

  • Data for Human IL-12 p70 ELISA Ready-SET-Go!®.
Description

Description: This Human IL-12 p70 ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This Human IL-12 p70 ELISA Ready-SET-Go! reagent set specifically measures the bioactive, heterodimeric form of IL-12, p70, without interference by p40 monomer, homodimer, or IL-23 (p19/p40). This ELISA reagent set is specifically engineered for accurate and precise measurement of human IL-12 p70 protein levels from samples including serum, plasma, and supernatants from cell cultures.

Details
Reactivity Human
Sensitivity 4 pg/ml
Standard Curve Range 4 - 500 pg/ml
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References

References: Fricke I, Mitchell D, Petersen F, Böhle A, Bulfone-Paus S, Brandau S. Platelet factor 4 in conjunction with IL-4 directs differentiation of human monocytes into specialized antigen-presenting cells. FASEB J. 2004 Oct;18(13):1588-90. (RSG ELISA, TC sup, PubMed)

Langrish CL, Buddle JC, Thrasher AJ, Goldblatt D. Neonatal dendritic cells are intrinsically biased against Th-1 immune responses. Clin Exp Immunol. 2002 Apr;128(1):118-23. (RSG ELISA, TC sup, PubMed)

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