Human IL-12 (p70) FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human IL-12 p70 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect IL-12 p70 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A2.

Biologically active IL-12 is a disulfide-linked heterodimeric 70-kDa cytokine composed of a 35-kDa (p35) and a 40-kDa (p40) subunit. p35, a member of the IL-6 superfamily, is secreted in response to IFN gamma and agonists of TLR3, 4, or 7. However, p35 expression has been shown to be inhibited by Th2 cytokines and expressed at much lower levels than p40. Expression of p40 is regulated independently of p35. Moreover, p40 has been shown to be secreted as either a monomer or homodimer. Although each subunit alone does not possess IL-12 bioactivity, the p40 homodimer can bind to the IL-12 receptor and act as an antagonist to IL-12 p70. The receptor for this cytokine is composed of two subunits, IL-12R beta 1 and IL-12R beta 2, the latter of which is the signaling component of the receptor.

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