anti-human IL-12

Description: BMS152 (B-T21) recognizes natural and recombinant human IL-12A p35/p70 but not IL-12-p40. BMS 152 blocks IL-12 induced proliferation on PHA and IL-2 activated lymphoblasts.

Biologically active IL-12 is a disulfide-linked heterodimeric 70-kDa cytokine composed of a 35-kDa (p35) and a 40-kDa (p40) subunit. p35, a member of the IL-6 superfamily, is secreted in response to IFN gamma and agonists of TLR3, 4, or 7. However, p35 expression has been shown to be inhibited by Th2 cytokines and expressed at much lower levels than p40. Expression of p40 is regulated independently of p35. Moreover, p40 has been shown to be secreted as either a monomer or homodimer. Although each subunit alone does not possess IL-12 bioactivity, the p40 homodimer can bind to the IL-12 receptor and act as an antagonist to IL-12 p70. The receptor for this cytokine is composed of two subunits, IL-12R beta 1 and IL-12R beta 2, the latter of which is the signaling component of the receptor.

Citations: Vancheri,Carlo; Mastruzzo,Claudio; Tomaselli,Valerio; Sortino,Maria Angela; D'Amico,Leda; Bellistri,Guglielmo; Pistorio,Maria P.; Salinaro,Elisa Trovato; Palermo,Filippo; Mistretta,Antonino; Crimi,Nunzio. Normal Human Lung Fibroblasts Differently Modulate Interleukin-10 and Interleukin-12 Production by Monocytes . Implications for an Altered Immune Response in Pulmonary Chronic Inflammation. American Journal of Respiratory Cell and Molecular Biology 2001;25:592-599. (Link)