Human IL-10 Platinum ELISA

Description: The human IL-10 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-10. The human IL-10 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones.
Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.

Citations: Giavedoni,L.D.. Simultaneous detection of multiple cytokines and chemokines from nonhuman primates using luminex technology. Journal of Immunological Methods 2005;301:89-101. (Link)

Zhang,X.; Hei,P.; Deng,L.; Lin,J.. Interleukin-10 gene promoter polymorphisms and their protein production in peritoneal fluid in patients with endometriosis. Molecular Human Reproduction 2007;13:135-140. (Link)

Jasny,Edith; Eisenblatter,Martin; Matz-Rensing,Kerstin; Tenner-Racz,Klara; Tenbusch,Matthias; Schrod,Annette; Stahl-Hennig,Christiane; Moos,Verena; Schneider,Thomas; Racz,Paul; Uberla,Klaus; Kaup,Franz Josef; Ignatius,Ralf. IL-12-Impaired and IL-12-Secreting Dendritic Cells Produce IL-23 upon CD154 Restimulation. The Journal of Immunology 2008;180:6629-6639. (Link)

Chen,R.F.; Liu,J.W.; Yeh,W.T.; Wang,L.; Chang,J.C.; Yu,H.R.; Cheng,J.T.; Yang,K.D.. Altered T helper 1 reaction but not increase of virus load in patients with dengue hemorrhagic fever. FEMS Immunology and Medical Microbiology 2005;44:43-50. (Link)

Karth,Georg Delle; Buberl,Anton; Nikfardjam,Mariam; Meyer,Brigitte; Wollenek,Gregor; Grimm,Michael; Lassnigg,Andrea; Brannath,Werner; Hiesmayr,Michael; Heinz,Gottfried. Role of amiodarone on the systemic inflammatory response induced by cardiac surgery: proinflammatory actions: [Role de l'amiodarone sur la reaction inflammatoire systemique provoquee par la chirurgie cardiaque : actions pro-inflammatoires]. Canadian Journal of Anesthesia 2007;54:262-268. (Link)

Alcaraz,A.J.; Sancho,L.; Manzano,L.; Esquivel,F.; Carrillo,A.; Prieto,A.; Bernstein,E.D.; Alvarez-Mon,M.. Newborn patients exhibit an unusual pattern of interleukin 10 and interferon {gamma} serum levels in response to cardiac surgery. Journal of Thoracic and Cardiovascular Surgery 2002;123:451-458. (Link)

Kakazu,Eiji; Kanno,Noriatsu; Ueno,Yoshiyuki; Shimosegawa,Tooru. Extracellular Branched-Chain Amino Acids, Especially Valine, Regulate Maturation and Function of Monocyte-Derived Dendritic Cells. The Journal of Immunology 2007;179:7137-7146. (Link)

Westby,Michael; Vaughan,Andrew N.; Balotta,Claudia; Galli,Massimo; Clerici ,Mario; Dalgleish,Angus G.. Low CD4 counts rather than superantigenic-like effects account for differences in expressed T-cell receptor (TCR) repertoires between HIV-1 seropositive long-term non-progressors and individuals with progressive disease. British Journal of Haematology 1998;102:1187-1196. (Link)

Xu,Guangwu; Nie,Hong; Li,Ningli; Zheng,Wenxin; Zhang,Dongqing; Feng,Guozhang; Ni,Liqing; Xu,Rong; Hong,Jian; Zhang,Jingwu Z.. Role of osteopontin in amplification and perpetuation of rheumatoid synovitis. Journal of Clinical Investigation 2005;115:1060-1067. (Link)

Jimenez,Jose Luis; Punzon,Carmen; Navarro,Joaquin; Munoz-Fernandez,M.Angeles; Fresno,Manuel. Phosphodiesterase 4 Inhibitors Prevent Cytokine Secretion by T Lymphocytes by Inhibiting Nuclear Factor-kappa B and Nuclear Factor of Activated T Cells Activation. Journal of Pharmacology and Experimental Therapeutics 2001;299:753-759. (Link)

de Lalla,Fausto; Nicolin,Roberto; Lazzarini,Luca. Safety and efficacy of recombinant granulocyte colony-stimulating factor as an adjunctive therapy for Streptococcus pneumoniae meningitis in non-neutropenic adult patients: a pilot study. The Journal of Antimicrobial Chemotherapy 2000;46:843-846. (Link)

Clerici,M.; Balotta,C.; Salvaggio,A.; Riva,C.; Trabattoni,D.; Papagno,L.; Berlusconi,A.; Rusconi,S.; Villa,M.L.; Moroni,M.; Galli,M.. Human immunodeficiency virus (HIV) phenotype and interleukin-2/ interleukin-10 ratio are associated markers of protection and progression in HIV infection. Blood 1996;88:574-579. (Link)

MARZI,M.; VIGANO,A.; Trabattoni,D.; Villa,M.L.; Salvaggio,A.; CLERICI,E.; Clerici,M.. Characterization of type 1 and type 2 cytokine production profile in physiologic and pathologic human pregnancy. Clinical and Experimental Immunology 1996;106:127-133. (Link)

Lacomba,Manuel Santos; Martin,Carmen Marcos; Chamond,Rafael Ramirez; Galera,Jose Maria Gallardo; Omar,Mohamed; Estevez,Eduardo Collantes. Aqueous and Serum Interferon {gamma}, Interleukin (IL) 2, IL-4, and IL-10 in Patients With Uveitis. Archives of Ophthalmology 2000;118:768-772. (Link)

Rico,M.A.; Quiroga,J.A.; Subira,D.; Garcia,E.; Castanon,S.; Sallberg,M.; Leroux-Roels,G.; Weiland,O.; Pardo,M.; Carreno,V.. Features of the CD4^+ T-cell response in liver and peripheral blood of hepatitis C virus-infected patients with... Journal of Hepatology 2002;36:408-416. (Link)

ALLENDE; CORELL; MANZANARES; MADRUGA; MARCOS; MADRONO; LOPEZ,G.O.Y.A.; GARCIA,P.E.R.E.; MORENO; RODRIGO; SANZ; ARNAIZ,V.I.L.L.. Immunodeficiency associated with anorexia nervosa is secondary and improves after refeeding. Immunology 1998;94:543-551. (Link)

Resino,S.; Bellon,J.Ma; Sanchez-Ramon,S.; Gurbindo,D.; Munoz-Fernandez,Ma A.. Clinical Relevance of Cytokine Production in HIV-1 Infection in Children on Antiretroviral Therapy. Scandinavian Journal of Immunology 2000;52:634-640. (Link)

Navarro,Joaquin; Punzon,Carmen; Jimenez,Jose Luis; Fernandez-Cruz,Eduardo; Pizarro,Angel; Fresno,Manuel; Munoz-Fernandez,M.Angeles. Inhibition of Phosphodiesterase Type IV Suppresses Human Immunodeficiency Virus Type 1 Replication and Cytokine Production in Primary T Cells: Involvement of NF-kappa B and NFAT. Journal of Virology 1998;72:4712-4720. (Link)

Bocsi,J¢zsef; Hambsch,J”rg; Osmancik,Pavel; Schneider,Peter; Valet,Gnter; T rnok,Attila. Preoperative prediction of pediatric patients with effusions and edema following cardiopulmonary bypass surgery by serological and routine laboratory data. Critical Care 2002;6:226-233. (Link)

Fiane,Arnt E.; Videm,Vibeke; Lingaas,Per S.; Heggelund,Lars; Nielsen,Erik W.; Geiran,Odd R.; Fung,Michael; Mollnes,Tom E.. Mechanism of Complement Activation and Its Role in the Inflammatory Response After Thoracoabdominal Aortic Aneurysm Repair. Circulation 2003;108:849-856. (Link)

TEUNISSEN,M.B.M.; KOOMEN,C.W.; JANSEN,J.; WAAL MALEFYT,R.; SCHMITT,E.; VAN DEN WIJNGAARD,R.M.J.G.; DAS,P.K.; BOS,J.D.. In contrast to their murine counterparts, normal human keratinocytes and human epidermoid cell lines A431 and HaCaT fail to express IL-10 mRNA and protein. Clinical and Experimental Immunology 1997;107:213-223. (Link)

Monari,Claudia; Kozel,Thomas R.; Paganelli,Francesca; Pericolini,Eva; Perito,Stefano; Bistoni,Francesco; Casadevall,Arturo; Vecchiarelli,Anna. Microbial Immune Suppression Mediated by Direct Engagement of Inhibitory Fc Receptor. The Journal of Immunology 2006;177:6842-6851. (Link)

Chen,Rong Fu; Liu,Jien Wei; Yeh,Wen Ting; Wang,Lin; Chang,Jen Chieh; Yu,Hong Ren; Cheng,Jiin Tsuey; Yang,Kuender D.. Altered T helper 1 reaction but not increase of virus load in patients with dengue hemorrhagic fever. FEMS Immunology and Medical Microbiology 2005;44:43-50. (Link)

Del Rey,Manuel; Ruiz-Contreras,Jesus; Bosque,Alberto; Calleja,Sara; Gomez-Rial,Jose; Roldan,Ernesto; Morales,Pablo; Serrano,Antonio; Anel,Alberto; Paz-Artal,Estela; Allende,Luis M.. A homozygous Fas ligand gene mutation in a patient causes a new type of autoimmune lymphoproliferative syndrome. Blood 2006;108:1306-1312. (Link)

Ishizuka; Okayama; Kobayashi; Mori. Interleukin-10 is localized to and released by human lung mast cells. Clinical & Experimental Allergy 1999;29:1424-1432. (Link)

References: Heinzel,F.P.; Sadick,M.D.; Mutha,S.S.; Locksley,R.M.. Production of interferon gamma, interleukin 2, interleukin 4, and interleukin 10 by CD4+ lymphocytes in vivo during healing and progressive murine leishmaniasis. Proc.Natl.Acad.Sci.U.S.A 1991;88:7011-7015. (Link)

Barnes,P.F.; Chatterjee,D.; Abrams,J.S.; Lu,S.; Wang,E.; Yamamura,M.; Brennan,P.J.; Modlin,R.L.. Cytokine production induced by Mycobacterium tuberculosis lipoarabinomannan. Relationship to chemical structure. J.Immunol. 1992;149:541-547. (Link)

Kim,J.M.; Brannan,C.I.; Copeland,N.G.; Jenkins,N.A.; Khan,T.A.; Moore,K.W.. Structure of the mouse IL-10 gene and chromosomal localization of the mouse and human genes. J.Immunol. 1992;148:3618-3623. (Link)

Mosier,D.E.; Yetter,R.A.; Morse,H.C.,III. Retroviral induction of acute lymphoproliferative disease and profound immunosuppression in adult C57BL/6 mice. J.Exp.Med. 1985;161:766-784. (Link)

Basu,S.; Dunn,A.; Ward,A.. G-CSF: function and modes of action (Review). Int.J.Mol.Med. 2002;10:3-10. (Link)

Vieira,P.; de Waal-Malefyt,R.; Dang,M.N.; Johnson,K.E.; Kastelein,R.; Fiorentino,D.F.; deVries,J.E.; Roncarolo,M.G.; Mosmann,T.R.; Moore,K.W.. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI. Proc.Natl.Acad.Sci.U.S.A 1991;88:1172-1176. (Link)

Moore,K.W.; Vieira,P.; Fiorentino,D.F.; Trounstine,M.L.; Khan,T.A.; Mosmann,T.R.. Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI. Science 1990;248:1230-1234. (Link)

Spits,H.; de Waal,Malefyt R.. Functional characterization of human IL-. Int.Arch.Allergy Immunol. 1992;99:8-15. (Link)

Kullberg,M.C.; Pearce,E.J.; Hieny,S.E.; Sher,A.; Berzofsky,J.A.. Infection with Schistosoma mansoni alters Th1/Th2 cytokine responses to a non-parasite antigen. J.Immunol. 1992;148:3264-3270. (Link)

de Waal,Malefyt R.; Abrams,J.; Bennett,B.; Figdor,C.G.; de Vries,J.E.. Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes. J.Exp.Med. 1991;174:1209-1220. (Link)

Moore,K.W.; O'Garra,A.; de Waal,Malefyt R.; Vieira,P.; Mosmann,T.R.. Interleukin-10. Annu.Rev.Immunol. 1993;11:165-190. (Link)

Shanafelt,A.B.; Miyajima,A.; Kitamura,T.; Kastelein,R.A.. The amino-terminal helix of GM-CSF and IL-5 governs high affinity binding to their receptors. EMBO J. 1991;10:4105-4112. (Link)

Baer,R.; Bankier,A.T.; Biggin,M.D.; Deininger,P.L.; Farrell,P.J.; Gibson,T.J.; Hatfull,G.; Hudson,G.S.; Satchwell,S.C.; Seguin,C.; .. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 1984;310:207-211. (Link)

Silva,J.S.; Morrissey,P.J.; Grabstein,K.H.; Mohler,K.M.; Anderson,D.; Reed,S.G.. Interleukin 10 and interferon gamma regulation of experimental Trypanosoma cruzi infection. J.Exp.Med. 1992;175:169-174. (Link)

Bloom,B.R.; Mehra,V.. Immunological unresponsiveness in leprosy. Immunol.Rev. 1984;80:5-28. (Link)