Human IL-1beta High Sensitivity ELISA

Description: This High Sensitivity ELISA enables the analysis of Interleukin-1beta in low concentrations from serum, plasma, or cell culture samples.

eBioscience High Sensitivity ELISA Kits offer accurate quantification below conventional ELISA limits. This is made possible by ther inherent design by amplifying the signal generated by the enzyme horseradish peroxidase (HRP) and the colorimetric-detectable substrate tetramethylbenzidin (TMB) based on Tyramide Signal Amplification (TSA) technology*.

IL-1beta is a pro-inflamatory cytokine and is expressed by many cells including macrophage, NK cells, monocytes, and neutrophils. It belongs to the IL-1 family cluster. Associated activities with IL-1 are the induction of Prostaglandin E2 by synovial cells and release of collagenase with resulting destruction of cartilage and bone resorption. IL-1, has multiple immunological functions including enhancement of IL-2 production by T cells and activation of B-cells (BAF) and thymocytes. The two distinct molecular forms of IL-1 are thought to be derived from two genes. After transcription, as 31 kD precursor polypeptide is cleaved to give rise to mostly cell membrane associated IL-1 alpha and secreted IL-1 beta. Both have the same molecular weight of 15 kD but have different isoelectric points of 5 and 7, respectively. Despite sequence homology of only 20%, both forms are thought to bind to the same receptor. IL-1 inhibitors that vary only in their degree of glycosylation have been described to bind to the IL-1 receptor. These inhibitors are structurally related to IL-1b and may be important in regulation of IL-1b action. Low levels of IL-1 beta have been reported in normal serum. It is thought that IL-1 genes are induced to respond to tissue damage or in infection. Elevated levels have been reported in a number of infectious disease conditions and in noninfectious inflammatory conditions such as Crohn's disease. In addition to elevated serum levels, IL-1 has been found in synovial fluids of patients with rheumatoid arthritis and in cerebrospinal fluid after neurological inflammation or insult. At the other end of the spectrum, low levels of IL-1 have been found in malnutrition and advanced neoplasia suggesting perhaps a complex immunological and physiological regulatory role for this cytokine.

*High Sensitivity ELISAs are based on a patent from PerkinElmer LAS, patent number 5,731,158; 5,583,001; 5,196,306.

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