Anti-Human IgM PE

Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.

Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.

References: Szyszko EA, Brun JG, Skarstein K, Peck AB, Jonsson R, Brokstad KA. Phenotypic diversity of peripheral blood plasma cells in primary Sjögren's syndrome. Scand J Immunol. 2011 Jan;73(1):18-28. (SA-DA4, FC)

Pack M, Trumpfheller C, Thomas D, Park CG, Granelli-Piperno A, Münz C, Steinman RM. DEC-205/CD205+ dendritic cells are abundant in the white pulp of the human spleen, including the border region between the red and white pulp. Immunology. 2008 Mar;123(3):438-46. (SA-DA4, IHC-fr)