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Human IgG total ELISA Ready-SET-Go!®

Also known as: Immunoglobulin G

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 88-50550

Cat. No. Size
88-50550-22 2 x 96 tests w/plates
88-50550-88 10 x 96 tests
88-50550-86 10 x 96 tests w/plates
88-50550-77 20 x 96 tests
88-50550-76 20 x 96 tests w/plates

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Description: IgG is the major immunoglobulin in blood, lymph fluid, cerebrospinal fluid, and peritoneal fluid and a key player in the humoral immune response. Serum IgG in healthy humans presents approximately 15% of total protein beside albumins, enzymes, other globulins and many more.

The Fc portion of IgG, but not F(ab')2 or Fab fragments, can cross the placenta of a mother to enter the fetal circulation providing the fetus with postpartum protection.

IgG molecules are able to react with Fc-gamma receptors that are present on the surfaces of macrophages, neutrophils, natural killer cells, and can activate the complement system.

The binding of the Fc portion of IgG to the receptor present on a phagocyte is a critical step in the opsonizing property IgG provides to the immune response. Phagocytosis of particles coated with IgG antibodies is a vital mechanism to cope with microorganisms.

IgG is produced in a delayed response to an infection and can be retained in the body for a long time. The longevity in serum makes IgG most useful for passive immunization by transfer of this antibody. Detection of IgG usually indicates a prior infection or vaccination.

Reactivity Human
Sample Volume 100 ul (prediluted 1:500,000)
Suitable Sample Types Cell culture supernatant, serum and plasma
Sensitivity 1.6 ng/ml
Standard Curve Range 1.6 - 100 ng/ml
Components Capture Antibody Concentrate
Detection Antibody Concentrate
Human IgG isotype control (standard), lyophilized, 200 ng/ml upon reconstitution
Phosphate Buffered Saline Concentrate (PBS)
Assay Buffer A Concentrate (PBS with 1% Tween 20 and 10% BSA)
Tetramethylbenzidine (TMB) Substrate Solution
96-well plates included with product Cat. #'s ending in suffixes -22, -76, -86
Reported Applications ELISA
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
39-50550 Human IgG total ELISA Ready-SET-Go!® Single-Use RSG ELISA Standard ELISA RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
88-50400 Mouse IgG total ELISA Ready-SET-Go! ELISA RUO
88-50620 Human IgM ELISA Ready-SET-Go!® ELISA RUO
88-50540 Rat IgM ELISA Ready-SET-Go!® ELISA RUO

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Area of BiologyIsotype Controls