Human IFN gamma ELISA Ready-SET-Go!®

Also known as: Interferon-gamma, IFN-g, IFNg

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-7316

Cat. No. Size
88-7316-22 2 x 96 tests
88-7316-88 10 x 96 tests
88-7316-86 10 x 96 tests w/plates
88-7316-77 20 x 96 tests
88-7316-76 20 x 96 tests w/plates

To enable ordering, please select your country. Cookies will need to be enabled to continue on our website. View our Privacy Policy.

Data for Human IFN gamma ELISA Ready-SET-Go!®.

Click on image to view more information.

More Views

  • Data for Human IFN gamma ELISA Ready-SET-Go!®.
Description

Description: This Human IFN-γ ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of human IFN-γ protein levels from samples including serum, plasma, and supernatants from cell cultures.

Special Note: To ensure optimal results from the Human IFNg ELISA Ready-SET-Go! Set, please only use the components included in the set. Exchanging of components is not recommended as a change in signal may occur.

Details
Reactivity Human
Sensitivity 4 pg/ml
Standard Curve Range 4 - 500 pg/ml
Components Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Reported Applications ELISA
Documentation

For complete product information, please download the TDS or IFU document.

TDS Link Download TDS
Additional Formats
References

References: Gupta AK, Holzgreve W, and Hahn W. 2005. Microparticle-free placentally derived soluble factors downmodulate the response of activated T cells. Hum Immunol. 66(9):977-84. (RSG ELISA kit, TC supernatant, PubMed)

Tao X, Li C, et al. 2007. Simultaneous detection of IFN-gamma and IL-4 in lesional tissues and whole unstimulated saliva from patients with oral lichen planus. J Oral Pathol Med. (RSG ELISA kit, Epub)

Horita K, Yoshitomi H, et al. 2007. Preferential recruitment of CCR6-expressing Th17 cells to inflamed joints via CCL20 in rheumatoid arthritis and its animal model. J Exp Med 204(12):2803-12. (RSG ELISA kit, synovial fluid, PubMed)

Gupta AK, Rusterholz C, et al. 2005. Constant IFNgamma mRNA to protein ratios in cord and adult blood T cells suggests regulation of IFNgamma expression in cord blood T cells occurs at the transcriptional level. Clin Exp Immunol 140(2):282-8. (RSG ELISA kit, TC supernatant and protein lysate, PubMed)