Human IFN-gamma High Sensitivity ELISA

Description: The human IFN-gamma ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IFN-gamma. The human IFN-gamma ELISA is for research use only. Not for diagnostic or therapeutic procedures.

IFN-gamma, also called Type II interferon, is a homodimeric glycoprotein containing approximately 21 to 24 kD subunits. The human IFN-gamma gene, situated on chromosome 12, contains three introns; the four exons code for a polypeptide of 166 amino acids, 20 of which constitute the signal peptide. In contrast to IFN-alpha and IFN-beta synthesis, which can occur in any cell, production of IFN-gamma is a function of T cells and NK cells.

IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. IFN-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types.

Citations: Allende,Luis M.; Lopez-Goyanes,Alberto; Paz-Artal,Estela; Corell,Alfredo; Garcia-Perez,M.Angel; Varela,Pilar; Scarpellini,Atilio; Negreira,Sagrario; Palenque,Elia; Arnaiz-Villena,Antonio. A Point Mutation in a Domain of Gamma Interferon Receptor 1 Provokes Severe Immunodeficiency. Clinical and Diagnostic Laboratory Immunology 2001;8:133-137. (Link)

Kondrusik,M.; Swierzbinska,R.; Pancewicz,S.; Zajkowska,J.; Grygorczuk,S.; Hermanowska-Szpakowicz,T.. Evaluation of proinflammatory cytokine (TNF-alpha, IL-1beta, IL-6, IFN-gamma) concentrations in serum and cerebrospinal fluid of patients with neuroborreliosis. Neurol Neurochir Pol. 2004;38:265-270. (Link)

Bellone,Graziella; Carbone,Anna; Smirne,Carlo; Scirelli,Tiziana; Buffolino,Alessandra; Novarino,Anna; Stacchini,Alessandra; Bertetto,Oscar; Palestro,Giorgio; Sorio,Claudio; Scarpa,Aldo; Emanuelli,Giorgio; Rodeck,Ulrich. Cooperative Induction of a Tolerogenic Dendritic Cell Phenotype by Cytokines Secreted by Pancreatic Carcinoma Cells. The Journal of Immunology 2006;177:3448-3460. (Link)

Sun,Yong Jiang; Lim,T.K.; Ong,Adrian Kheng Yeow; Ho,Benjamin Choon Heng; Seah,Geok Teng; Paton,Nicholas I.. Tuberculosis associated with Mycobacterium tuberculosis Beijing and non-Beijing genotypes: a clinical and immunological comparison. BMC Infectious Diseases 2006;6:105(Link)

SOLERTE,S.B.; Ceresini,G.; FERRARI,E.; FIORAVANTI,M.. Hemorheological changes and overproduction of cytokines from immune cells in mild to moderate dementia of the... Neurobiology of Aging 2000;21:271-281. (Link)

Vigano,P.; Gaffuri,B.; Somigliana,E.; Infantino,M.; Vignali,M.; Di Blasio,A.M.. Interleukin-10 is produced by human uterine natural killer cells but does not affect their production of interferon-{gamma}. Molecular Human Reproduction 2001;7:971-977. (Link)

Paccani,Silvia Rossi; Tonello,Fiorella; Ghittoni,Raffaella; Natale,Mariarita; Muraro,Lucia; D'Elios,Mario Milco; Tang,Wei Jen; Montecucco,Cesare; Baldari,Cosima T.. Anthrax toxins suppress T lymphocyte activation by disrupting antigen receptor signaling. Journal of Experimental Medicine 2005;201:325-331. (Link)

Chen,R.F.; Liu,J.W.; Yeh,W.T.; Wang,L.; Chang,J.C.; Yu,H.R.; Cheng,J.T.; Yang,K.D.. Altered T helper 1 reaction but not increase of virus load in patients with dengue hemorrhagic fever. FEMS Immunology and Medical Microbiology 2005;44:43-50. (Link)

Mukai,Harumi Y.; Hasegawa,Yuichi; Kojima,Hiroshi; Okoshi,Yasushi; Takei,Naoko; Yamashita,Yoriko; Nagasawa,Toshiro; Mori,Naoyoshi. Nodal CD8 Positive Cytotoxic T-Cell Lymphoma: A Distinct Clinicopathological Entity. Modern Pathology 2002;15:1131-1139. (Link)

Yamada,Takashi; Sato,Akira; Komiya,Ichiro; Nishimori,Takeo; Ito,Yoshiharu; Terao,Akira; Eto,Sumiya; Tanaka,Yoshiya. An Elevation of Serum Immunoglobulin E Provides a New Aspect of Hyperthyroid Graves' Disease. Journal of Clinical Endocrinology & Metabolism 2000;85:2775-2778. (Link)

SOLERTE,S.B.; CRAVELLO,L.; FERRARI,E.; FIORAVANTI,M.. Overproduction of IFN-gamma and TNF-alpha from Natural Killer (NK) Cells Is Associated with Abnormal NK Reactivity and Cognitive Derangement in Alzheimer's Disease. Annals of the New York Academy of Sciences 2000;917:331-340. (Link)

Schlaepfer,Erika; Audige,Annette; Joller,Helene; Speck,Roberto F.. TLR7/8 Triggering Exerts Opposing Effects in Acute versus Latent HIV Infection. The Journal of Immunology 2006;176:2888-2895. (Link)

Schena,Francesco; Cerullo,Giuseppina; Torres,Diletta; Scolari,Francesco; Foramitti,Marina; Amoroso,Antonio; Pirulli,Doroti; Floege,Jurgen; Mertens,Peter; Zerres,Klaus; Alexopoulos,Efstathios; Kirmizis,Dimitrios; Zelante,Leopoldo; Bisceglia,Luigi. Role of interferon-[gamma] gene polymorphisms in susceptibility to IgA nephropathy: a family-based association study. Eur J Hum Genet 2006;14:488-496. (Link)

Xu,Guangwu; Nie,Hong; Li,Ningli; Zheng,Wenxin; Zhang,Dongqing; Feng,Guozhang; Ni,Liqing; Xu,Rong; Hong,Jian; Zhang,Jingwu Z.. Role of osteopontin in amplification and perpetuation of rheumatoid synovitis. Journal of Clinical Investigation 2005;115:1060-1067. (Link)

Ohno,Ryuzo; Yamaguchi,Yoshiyuki; Toge,Tetsuya; Kinouchi,Toshiaki; Kotake,Toshihiko; Shibata,Masahiko; Kiyohara,Yoshio; Ikeda,Shigeo; Fukui,Iwao; Gohchi,Akira; Sugiyama,Yasuyuki; Saji,Shigetoyo; Hazama,Shouichi; Oka,Masaaki; Ohnishi,Kazunori; Ohhashi,Yasuo; Tsukagoshi,Shigeru; Taguchi,Tetsuo. A Dose-Escalation and Pharmacokinetic Study of Subcutaneously Administered Recombinant Human Interleukin 12 and Its Biological Effects in Japanese Patients with Advanced Malignancies. Clinical Cancer Research 2000;6:2661-2669. (Link)

Ben-Asouli,Y.; Banai,Y.; Pel-Or,Y.; Shir,A.; Kaempfer,R.. Human Interferon-. Cell 2002;108:221-232. (Link)

Lacomba,Manuel Santos; Martin,Carmen Marcos; Chamond,Rafael Ramirez; Galera,Jose Maria Gallardo; Omar,Mohamed; Estevez,Eduardo Collantes. Aqueous and Serum Interferon {gamma}, Interleukin (IL) 2, IL-4, and IL-10 in Patients With Uveitis. Archives of Ophthalmology 2000;118:768-772. (Link)

ALLENDE; CORELL; MANZANARES; MADRUGA; MARCOS; MADRONO; LOPEZ,G.O.Y.A.; GARCIA,P.E.R.E.; MORENO; RODRIGO; SANZ; ARNAIZ,V.I.L.L.. Immunodeficiency associated with anorexia nervosa is secondary and improves after refeeding. Immunology 1998;94:543-551. (Link)

Schonbeck,Uwe; Gerdes,Norbert; Varo,Nerea; Reynolds,Rebecca S.; Horton,Daniel B.; Bavendiek,Udo; Robbie,Linda; Ganz,Peter; Kinlay,Scott; Libby,Peter. Oxidized Low-Density Lipoprotein Augments and 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Inhibitors Limit CD40 and CD40L Expression in Human Vascular Cells. Circulation 2002;106:2888-2893. (Link)

FERRARI,E.; FIORAVANTI,M.; MAGRI,F.; SOLERTE,S.B.. Variability of Interactions Between Neuroendocrine and Immunological Functions in Physiological Aging and Dementia of the Alzheimer's Type. Annals of the New York Academy of Sciences 2000;917:582-596. (Link)

Xiao,J.Z.; Kondo,S.; Yanagisawa,N.; Takahashi,N.; Odamaki,T.; Iwabuchi,N.; Miyaji,K.; Iwatsuki,K.; Togashi,H.; Enomoto,K.; Enomoto,T.. Probiotics in the treatment of Japanese cedar pollinosis: a double-blind placebo-controlled trial. Clinical & Experimental Allergy 2006;36:1425-1435. (Link)

Ogawa,Tomohiko; Hashikawa,Shinnosuke; Asai,Yasuyuki; Sakamoto,Hiromi; Yasuda,Kenji; Makimura,Yutaka. A new synbiotic, Lactobacillus casei subsp. casei together with dextran, reduces murine and human allergic reaction. FEMS Immunology and Medical Microbiology 2006;46:400-409. (Link)

Juvonen,Jukka; Surcel,Helja Marja; Satta,Jari; Teppo,Anna Maija; Bloigu,Aini; Syrjala,Hannu; Airaksinen,Juhani; Leinonen,Maija; Saikku,Pekka; Juvonen,Tatu. Elevated Circulating Levels of Inflammatory Cytokines in Patients With Abdominal Aortic Aneurysm. Arteriosclerosis, Thrombosis, and Vascular Biology 1997;17:2843-2847. (Link)

Scheffer,C.; Zawatzky,R.; Rink,L.. Interferon-. Mechanisms of Ageing and Development 2001;121:47-58. (Link)

Chen,Rong Fu; Liu,Jien Wei; Yeh,Wen Ting; Wang,Lin; Chang,Jen Chieh; Yu,Hong Ren; Cheng,Jiin Tsuey; Yang,Kuender D.. Altered T helper 1 reaction but not increase of virus load in patients with dengue hemorrhagic fever. FEMS Immunology and Medical Microbiology 2005;44:43-50. (Link)

References: Cunningham,A.L.; Nelson,P.A.; Fathman,C.G.; Merigan,T.C.. Interferon gamma production by herpes simplex virus antigen-specific T cell clones from patients with recurrent herpes labialis. J.Gen.Virol. 1985;66 ( Pt 2):249-258. (Link)

Sidman,C.L.; Marshall,J.D.; Shultz,L.D.; Gray,P.W.; Johnson,H.M.. Gamma-interferon is one of several direct B cell-maturing lymphokines. Nature 1984;309:801-804. (Link)

Nast,C.C.; Zuo,X.J.; Prehn,J.; Danovitch,G.M.; Wilkinson,A.; Jordan,S.C.. Gamma-interferon gene expression in human renal allograft fine-needle aspirates. Transplantation 1994;57:498-502. (Link)

Mosmann,T.R.; Cherwinski,H.; Bond,M.W.; Giedlin,M.A.; Coffman,R.L.. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J.Immunol. 1986;136:2348-2357. (Link)

Suomalainen,H.; Soppi,E.; Laine,S.; Isolauri,E.. Immunologic disturbances in cow's milk allergy, 2: Evidence for defective interferon-gamma generation. Pediatr.Allergy Immunol. 1993;4:203-207. (Link)

Davidson,P.M.; Creati,L.; Wood,P.R.; Roberton,D.M.; Hosking,C.S.. Lymphocyte production of gamma-interferon as a test for non-tuberculous mycobacterial lymphadenitis in childhood. Eur.J.Pediatr. 1993;152:31-35. (Link)

Naylor,S.L.; Sakaguchi,A.Y.; Shows,T.B.; Law,M.L.; Goeddel,D.V.; Gray,P.W.. Human immune interferon gene is located on chromosome 12. J.Exp.Med. 1983;157:1020-1027. (Link)

Tang,M.L.; Kemp,A.S.; Thorburn,J.; Hill,D.J.. Reduced interferon-gamma secretion in neonates and subsequent atopy. Lancet 1994;344:983-985. (Link)

Aoki,Y.; Katoh,O.; Nakanishi,Y.; Kuroki,S.; Yamada,H.. A comparison study of IFN-gamma, ADA, and CA125 as the diagnostic parameters in tuberculous pleuritis. Respir.Med. 1994;88:139-143. (Link)

Ciampolillo,A.; Guastamacchia,E.; Caragiulo,L.; Lollino,G.; De,Robertis O.; Lattanzi,V.; Giorgino,R.. In vitro secretion of interleukin-1 beta and interferon-gamma by peripheral blood lymphomononuclear cells in diabetic patients. Diabetes Res.Clin.Pract. 1993;21:87-93. (Link)

Olsson,T.. Multiple sclerosis:cerebrospinal fluid. Ann.Neurol. 1994;36 Suppl:S100-S102. (Link)

Shinde,S.R.; Chiplunkar,S.V.; Butlin,R.; Samson,P.D.; Deo,M.G.; Gangal,S.G.. Lymphocyte proliferation, IFN-gamma production and limiting dilution analysis of T-cell responses to ICRC and Mycobacterium leprae antigens in leprosy patients. Int.J.Lepr.Other Mycobact.Dis. 1993;61:51-58. (Link)

Capobianchi,M.R.; Ameglio,F.; Tosi,R.; Dolei,A.. Differences in the expression and release of DR, BR, and DQ molecules in human cells treated with recombinant interferon gamma: comparison to other interferons. Hum.Immunol. 1985;13:1-11. (Link)

Edwards,B.S.; Merritt,J.A.; Fuhlbrigge,R.C.; Borden,E.C.. Low doses of interferon alpha result in more effective clinical natural killer cell activation. J.Clin.Invest 1985;75:1908-1913. (Link)

Le,thi Bich-Thuy; Queen,C.; Fauci,A.S.. Interferon-gamma induces light chain synthesis in interleukin 2 stimulated human B cells. Eur.J.Immunol. 1986;16:547-550. (Link)

Fitch,F.W.; McKisic,M.D.; Lancki,D.W.; Gajewski,T.F.. Differential regulation of murine T lymphocyte subsets 40. Annu.Rev.Immunol. 1993;11:29-48. (Link)

Sampaio,E.P.; Kaplan,G.; Miranda,A.; Nery,J.A.; Miguel,C.P.; Viana,S.M.; Sarno,E.N.. The influence of thalidomide on the clinical and immunologic manifestation of erythema nodosum leprosum. J.Infect.Dis. 1993;168:408-414. (Link)

Urban,J.L.; Shepard,H.M.; Rothstein,J.L.; Sugarman,B.J.; Schreiber,H.. Tumor necrosis factor: a potent effector molecule for tumor cell killing by activated macrophages. Proc.Natl.Acad.Sci.U.S.A 1986;83:5233-5237. (Link)

Sastre,L.; Roman,J.M.; Teplow,D.B.; Dreyer,W.J.; Gee,C.E.; Larson,R.S.; Roberts,T.M.; Springer,T.A.. A partial genomic DNA clone for the alpha subunit of the mouse complement receptor type 3 and cellular adhesion molecule Mac-1. Proc.Natl.Acad.Sci.U.S.A 1986;83:5644-5648. (Link)

Soliman,A.A.; el-Aggan,H.A.; el-Hefnawy,A.M.; Mahmoud,S.A.; bo Deya,S.H.. The value of ascites adenosine deaminase activity and interferon gamma level in discriminating tuberculous from non-tuberculous ascites. J.Egypt.Soc.Parasitol. 1994;24:93-105. (Link)