Anti-Human/Mouse beta-Catenin Alexa Fluor® 488

Clone: 15B8

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-2567

Cat. No.	Size
53-2567-41	25 tests
53-2567-42	100 tests
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Intracellular staining of the Jurkat cell line using the Foxp3 Staining Buffer Set (cat. 00-5523)...View More

Description

Description: The 15B8 monoclonal antibody reacts with human and mouse beta-catenin, one member of a family of catenins, which are intracellular proteins that interact with cadherins to mediate cellular adhesion. More specifically, beta-catenin binds to the cytoplasmic tail of E-cadherin. In addition, this molecule is a component of the canonical Wnt signaling pathway. In the absence of Wnt binding its receptor, beta-catenin is phosphorylated and resides in the cytoplasm where it is eventually targeted for degradation by ubiquitination. Upon Wnt binding, beta-catenin becomes dephosphorylated, translocates to the nucleus, and modulates gene expression in partnership with the transcription factors T cell factor (TCF) and lymphocyte enhancer binding factor (LEF). Expression of beta-catenin is found in a wide variety of non-immune and immune tissues, including thymocytes and T and B lymphocytes. The Wnt & beta-catenin signaling pathway has been demonstrated to play a crucial role in the development of T, B, and hematopoietic stem cells.

Details

Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human, Mouse
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
50-2567	Anti-Human/Mouse beta-Catenin eFluor® 660 (Alexa Fluor® 647 Replacement)	633 - 647 nm	668 nm	FC	RUO
14-2567	Anti-Human/Mouse beta-Catenin Purified			FC, IP, WB	RUO
12-2567	Anti-Human/Mouse beta-Catenin PE	488 - 561 nm		FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-5523	Foxp3 / Transcription Factor Staining Buffer Set			FC	GPR
53-4714	Mouse IgG1 K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Xu M, Sharma A, Hossain MZ, Wiest DL, Sen JM. Sustained expression of pre-TCR induced beta-catenin in post-beta-selection thymocytes blocks T cell development. J Immunol. 2009 Jan 15;182(2):759-65.

Staal FJ, Sen JM. The canonical Wnt signaling pathway plays an important role in lymphopoiesis and hematopoiesis. Eur J Immunol. 2008 Jul;38(7):1788-94. Review.

Xu, Y, Banerjee D, Huelsken J, Birchmeier W, and Sen JM. Deletion of beta-catenin impairs T cell development. Nat. Immunol. 2003 4:1177-1182.

Hoschuetzky H, Aberle H, Kemler R. Beta-catenin mediates the interaction of the cadherin-catenin complex with epidermal growth factor receptor. J Cell Biol. 1994 Dec;127(5):1375-80.

View More Products

Antigens	Area of Biology	Cell Type	Formats
Beta-Catenin	Stem Cell Biology Adhesion & Migration Angiogenesis Transcription Factors	B Cells T Cells	Alexa Fluor 488