Human G-CSF Instant ELISA

Description: The human G-CSF Instant ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human G-CSF. The human G-CSF Instant ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Granulocyte colony stimulating factor (G-CSF) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. The pleotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts and bone marrow cells. G-CSF has been shown to have specific effects on the proliferation, differentiation and activation of hematopoietic cells. G-CSF is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukaemia cells.
The protein is encoded by two distinct DNA sequences, resulting in a full size, high activity and a shorter, low activity isoform of G-CSF. The protein is highly conserved among species. G-CSF has been shown to exert its biological functions through interaction with its receptor expressed on the surface of hematopoietic progenitors and neutrophilic granulocytes as well as certain carcinoma cell lines. A soluble isoform of the G-CSF receptor has been described. Clinical use of G-CSF has been approved for several therapeutical applications, treatment of neonatal infections, therapy of acute myocardial infarction, granulocyte transfusion in patients with neutropenia, in severe infections and sepsis, therapy in chronic autoimmune neutropenia, treatment of acute myeloid leukemias, Sweet's syndrome and Aids.
G-CSF has further been shown to be a marker protein for different carcinomas such as bladder cancer.

Citations: Wojakowski,Wojciech; Tendera,Michal; Michalowska,Anna; Majka,Marcin; Kucia,Magdalena; Maslankiewicz,Katarzyna; Wyderka,Rafal; Ochala,Andrzej; Ratajczak,Mariusz Z.. Mobilization of CD34/CXCR4+, CD34/CD117+, c-met+ Stem Cells, and Mononuclear Cells Expressing Early Cardiac, Muscle, and Endothelial Markers Into Peripheral Blood in Patients With Acute Myocardial Infarction. Circulation 2004;110:3213-3220. (Link)

References: Rapoport,A.P.; Abboud,C.N.; DiPersio,J.F.. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF): receptor biology, signal transduction, and neutrophil activation. Blood Rev. 1992;6:43-57. (Link)

Reuss-Borst,M.A.; Muller,C.A.; Waller,H.D.. The possible role of G-CSF in the pathogenesis of Sweet's syndrome. Leuk.Lymphoma 1994;15:261-264. (Link)

Spiekermann,K.; Roesler,J.; Emmendoerffer,A.; Elsner,J.; Welte,K.. Functional features of neutrophils induced by G-CSF and GM-CSF treatment: differential effects and clinical implications. Leukemia 1997;11:466-478. (Link)

Wiedermann,F.J.; Mittermayr,M.; Hoffmann,G.; Schobersberger,W.. Recombinant granulocyte colony-stimulating factor (G-CSF) in infectious diseases: still a debate. Wien.Klin.Wochenschr. 2001;113:90-96. (Link)

Scarffe,J.H.; Kamthan,A.. Clinical studies of granulocyte colony stimulating factor (G-CSF). Cancer Surv. 1990;9:115-130. (Link)

Carr,R.; Modi,N.; Dore,C.. G-CSF and GM-CSF for treating or preventing neonatal infections. Cochrane.Database.Syst.Rev. 2003;CD003066(Link)

Tachibana,M.; Murai,M.. G-CSF production in human bladder cancer and its ability to promote autocrine growth: a review. Cytokines Cell Mol Ther. 1998;4:113-120. (Link)

Price,T.H.. Granulocyte transfusion in the G-CSF era. Int.J Hematol. 2002;76 Suppl 2:77-80. (Link)

Gabrilove,J.L.. Clinical applications of granulocyte colony stimulating factor (G-CSF). Growth Factors 1992;6:187-191. (Link)

Price,T.H.. The use of G-CSF in normal neutrophil donors. Ann Med Interne (Paris) 2000;151:115-122. (Link)

Cairo,M.S.. Review of G-CSF and GM-CSF. Effects on neonatal neutrophil kinetics. Am J Pediatr.Hematol.Oncol 1989;11:238-244. (Link)

Takano,H.; Ohtsuka,M.; Akazawa,H.; Toko,H.; Harada,M.; Hasegawa,H.; Nagai,T.; Komuro,I.. Pleiotropic effects of cytokines on acute myocardial infarction: G-CSF as a novel therapy for acute myocardial infarction. Curr.Pharm.Des 2003;9:1121-1127. (Link)

Tkatch,L.S.; Tweardy,D.J.. Human granulocyte colony-stimulating factor (G-CSF), the premier granulopoietin: biology, clinical utility, and receptor structure and function. Lymphokine Cytokine Res. 1993;12:477-488. (Link)

Tsuji,K.; Ebihara,Y.. Expression of G-CSF receptor on myeloid progenitors. Leuk.Lymphoma 2001;42:1351-1357. (Link)

Ruef,C.; Coleman,D.L.. [GM-CSF and G-CSF: cytokines in clinical application]. Schweiz.Med Wochenschr. 1991;121:397-412. (Link)

Visani,G.; Manfroi,S.. G-CSF in the biology and treatment of acute myeloid leukemias. Leuk.Lymphoma 1995;18:423-428. (Link)

Bauduer,F.. G-CSF: a very efficient therapy in chronic autoimmune neutropenia. A brief review of the literature. Hematol.Cell Ther. 1998;40:189-191. (Link)

Hubel,K.; Dale,D.C.; Engert,A.; Liles,W.C.. Use of G-CSF for granulocyte transfusion therapy. Cytokines Cell Mol Ther. 2000;6:89-95. (Link)