Human G-CSF FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human G-CSF in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect G-CSF alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A11.

Granulocyte colony stimulating factor (G-CSF) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. The pleotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts and bone marrow cells. G-CSF has been shown to have specific effects on the proliferation, differentiation and activation of hematopoietic cells. G-CSF is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukaemia cells.
The protein is encoded by two distinct DNA sequences, resulting in a full size, high activity and a shorter, low activity isoform of G-CSF. The protein is highly conserved among species. G-CSF has been shown to exert its biological functions through interaction with its receptor expressed on the surface of hematopoietic progenitors and neutrophilic granulocytes as well as certain carcinoma cell lines. A soluble isoform of the G-CSF receptor has been described. Clinical use of G-CSF has been approved for several therapeutical applications, treatment of neonatal infections, therapy of acute myocardial infarction, granulocyte transfusion in patients with neutropenia, in severe infections and sepsis, therapy in chronic autoimmune neutropenia, treatment of acute myeloid leukemias, Sweet?s syndrome and Aids.
G-CSF has further been shown to be a marker protein for different carcinomas such as bladder cancer.

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Tsuji,K.; Ebihara,Y.. Expression of G-CSF receptor on myeloid progenitors. Leuk.Lymphoma 2001;42:1351-1357. (Link)

Ruef,C.; Coleman,D.L.. [GM-CSF and G-CSF: cytokines in clinical application]. Schweiz.Med Wochenschr. 1991;121:397-412. (Link)

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Bauduer,F.. G-CSF: a very efficient therapy in chronic autoimmune neutropenia. A brief review of the literature. Hematol.Cell Ther. 1998;40:189-191. (Link)

Hubel,K.; Dale,D.C.; Engert,A.; Liles,W.C.. Use of G-CSF for granulocyte transfusion therapy. Cytokines Cell Mol Ther. 2000;6:89-95. (Link)