Human Regulatory T Cell Staining Kit #3

Also known as: Forkhead Box P3, Scurfin, JM2, Treg, Foxp3 PCH101 PE-Cy5; CD4 FITC; CD25 PE

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 88-8995

Cat. No.	Size
88-8995-40	Kit
Please inquire about custom configurations or bulk packaging.

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Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (cat. 11-0049) then fixed...View More

Description

Description: Regulatory T cells/Tregs (also known as suppressor T cells) are a specialized subpopulation of T cells that act to suppress immune responses thereby maintaining homeostasis and self tolerance. Intense investigation has tried to determine exclusive protein markers for Tregs. This has been complicated by the identification of four regulatory T cells subsets; natural, anergic, Tr1 and CD8+CD28- suppressor T cells. There are still no cell surface molecules that uniquely distinguish functional regulatory cells from conventional T cells. It has been proposed that the “natural” regulatory T cells originally recognized by their constitutive expression of CD4 and CD25 are further defined by expression of the transcription factor Foxp3. Additionally Foxp3 has also been characterized in CD8+CD28- suppressor T cells.

This kit has been optimized for staining normal hPBMCs.

The Human Regulatory T Cell Staining Kit contains components needed to stain the natural Tregs including CD4, CD25 and Foxp3.

Not included:
Isotype control for anti-CD4 and anti-CD25 (cocktail of FITC & PE mouse IgG1, cat. 22-2714)

Details

Reactivity	Human
Components	Cocktail of FITC anti-human CD4 (RPA-T4) and PE anti-human CD25 (BC96): 25 tests. Store at 2-8°C in the dark. PE-Cy5 anti-human Foxp3 (PCH101): 25 tests. Store at 2-8°C in the dark. PE-Cy5 Rat IgG2a isotype control: 25 µg. Store at 2-8°C in the dark. Normal Rat Serum: 100 µl. Store at 2-8°C. eBioscience Flow Cytometry Staining Buffer: 200 ml. Store at 2-8°C. eBioscience Fixation/Permeabilization Concentrate: 30 ml. Store at 2-8°C. Avoid agitation. This is a 4X stock solution that must be diluted prior to use with the Fixation/Permeabilization Diluent. Dilute 1 part Fixation/Permeabilization Concentrate with 3 parts Fixation/Permeabilization Diluent. Use within 6 months of receipt. Caution: This solution contains Paraformaldehyde, which is toxic and a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided. Wear proper protective clothing and gloves. eBioscience Fixation/Permeabilization Diluent: 100 ml. Store at 2-8°C. The diluent is intended to be used in combination with the Fixation/Permeabilization Concentrate. eBioscience Permeabilization Buffer (10X): 100 ml. Store at 2-8°C. Dilute to 1X with deionized/distilled water and store at 4°C. Caution: Harmful if swallowed or irritant by contact. Wear proper protective clothing and gloves. Note: The 10X Permeabilization Buffer has a natural tendency to precipitate, however, its function is not affected by this. To clarify, the solution can be filtered after dilution to 1X working solution.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-5523	Foxp3 / Transcription Factor Staining Buffer Set			FC	GPR
11-0049	Anti-Human CD4 FITC	488 nm	518 nm	FC	RUO
15-4321	Rat IgG2a K Isotype Control PE-Cyanine5	488 - 561 nm		FC	RUO
88-4999	Human/Non-Human Primate Regulatory T Cell Staining Kit #1			FC	RUO
88-8111	Mouse Regulatory T Cell Staining Kit #1			FC	RUO
88-8118	Mouse Regulatory T Cell Staining Kit #2			FC	RUO
88-8998	Human Regulatory T Cell Staining Kit #2			FC	RUO
88-8115	Mouse Regulatory T Cell Staining Kit #3			FC	RUO

References

References: Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK. 2005. Human CD4+ T Cells Express TLR5 and Its Ligand Flagellin Enhances the Suppressive Capacity and Expression of FOXP3 in CD4+CD25+ T Regulatory Cells. J Immunol. 2005 Dec 15;175(12):8051-9. (PCH101, intracellular flow, PubMed)

Lim, H.W., P. Hillsamer, A.H. Banham, and C.H. Kim. 2005. Cutting Edge: Direct Suppression of B cells by CD4+CD25+ Regulatory T cells. J. Immunol. 175: 4180-4183. (PCH101, intracellular flow, PubMed)

Seddiki N, Santner-Nanan B, Martinson J, Zaunders J, Sasson S, Landay A, Solomon M, Selby W, Alexander SI, Nanan R, Kelleher A, Fazekas de St Groth B. Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cells. J Exp Med. 2006 Jul 10;203(7):1693-700

Valencia X, Stephens G, Goldbach-Mansky R, Wilson M, Shevach EM, Lipsky PE.TNF down-modulates the function of human CD4+CD25hi T regulatory cells.Blood. 2006 Mar 14 [Intracellular staining for flow cytometry using PCH101]

View More Products

Antigens	Area of Biology	Cell Type
Foxp3	Adaptive Immunity Metabolism Transcription Factors	T Cells Treg Cells