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Anti-Human Foxp3 eFluor® 660

Also known as: Forkhead Box P3, Scurfin, JM2

Clone: PCH101

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 50-4776

Cat. No. Size
50-4776-41 25 tests
50-4776-42 100 tests

Data for Anti-Human Foxp3 eFluor® 660 (Alexa Fluor® 647 Replacement).

Staining of normal human peripheral blood cells with Anti-Human CD25 PE (cat. 12-0259), followed by ...View More

  • Data for Anti-Human Foxp3 eFluor® 660 (Alexa Fluor® 647 Replacement).

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259).

Host Rat
Isotype IgG2a, kappa
Reactivity Chimpanzee, Human, Rhesus
Conjugate eFluor 660
Laser Red Laser
Emit 668 nm
Excite 633 - 647 nm
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
12-4776 Anti-Human Foxp3 PE 488 - 561 nm 578 nm FC RUO
53-4776 Anti-Human Foxp3 Alexa Fluor® 488 488 nm 519 nm FC RUO
56-4776 Anti-Human Foxp3 Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
48-4776 Anti-Human Foxp3 eFluor® 450 405 nm 445 nm FC RUO
14-4776 Anti-Human Foxp3 Purified IHC-F, IHC-P, WB RUO
17-4776 Anti-Human Foxp3 APC 633 - 647 nm 660 nm FC RUO
11-4776 Anti-Human Foxp3 FITC 488 nm 520 nm FC, IHC RUO
15-4776 Anti-Human Foxp3 PE-Cyanine5 488 - 561 nm 667 nm FC RUO
25-4776 Anti-Human Foxp3 PE-Cyanine7 488 - 561 nm 775 nm FC RUO
61-4776 Anti-Human Foxp3 PE-eFluor® 610 488 - 561 nm 607 nm FC RUO
58-4776 Anti-Human Foxp3 Alexa Fluor® 532 532 nm 561 nm FC RUO
35-4776 Anti-Human Foxp3 PE-Cyanine5.5 488 - 567 nm 690 nm FC RUO
13-4776 Anti-Human Foxp3 Biotin FC, IHC-F, IHC-P, WB RUO
45-4776 Anti-Human Foxp3 PerCP-Cyanine5.5 488 nm 695 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-5523 Foxp3 / Transcription Factor Staining Buffer Set FC GPR
12-0259 Anti-Human CD25 PE 488 - 561 nm 578 nm FC RUO
00-5521 Foxp3 / Transcription Factor Fixation/Permeabilization Concentrate and Diluent FC GPR
11-0048 Anti-Human CD4 FITC 488 nm 520 nm FC RUO
50-4321 Rat IgG2a K Isotype Control eFluor® 660 633 - 647 nm 668 nm FC, ICC, IHC RUO

References: Paroni M, Magarotto A, Tartari S, Nizzoli G, Larghi P, Ercoli G, Gianelli U, Pagani M, Elli L, Abrignani S, Conte D, Geginat J, Caprioli F. Uncontrolled IL-17 Production by Intraepithelial Lymphocytes in a Case of non-IPEX Autoimmune Enteropathy. Clin Transl Gastroenterol. 2016 Jul 14;7(7):e182. (PCH101, FC, PubMed)

Rueda CM, Presicce P, Jackson CM, Miller LA, Kallapur SG, Jobe AH, Chougnet CA. Lipopolysaccharide-Induced Chorioamnionitis Promotes IL-1-Dependent Inflammatory FOXP3+ CD4+ T Cells in the Fetal Rhesus Macaque. J Immunol. 2016 May 1;196(9):3706-15. (PCH101, FC in rhesus, PubMed)

Blackburn MJ, Zhong-Min M, Caccuri F, McKinnon K, Schifanella L, Guan Y, Gorini G, Venzon D, Fenizia C, Binello N, Gordon SN, Miller CJ, Franchini G, Vaccari M. Regulatory and Helper Follicular T Cells and Antibody Avidity to Simian Immunodeficiency Virus Glycoprotein 120. J Immunol. 2015 Oct 1;195(7):3227-36. (PCH101, FC rhesus, PubMed)

Charbonnier LM, Janssen E, Chou J, Ohsumi TK, Keles S, Hsu JT, Massaad MJ, Garcia-Lloret M, Hanna-Wakim R, Dbaibo G, Alangari AA, Alsultan A, Al-Zahrani D, Geha RS, Chatila TA. Regulatory T-cell deficiency and immune dysregulation, polyendocrinopathy, enteropathy, X-linked-like disorder caused by loss-of-function mutations in LRBA. J Allergy Clin Immunol. 2015 Jan;135(1):217-27. (PCH101, FC, PubMed)

Bell MP, Svingen PA, Rahman MK, Xiong Y, Faubion WA Jr. FOXP3 regulates TLR10 expression in human T regulatory cells. J Immunol. 2007 Aug 1;179(3):1893-900. (PCH101, ChIP assay, PubMed)

Clark RA, Kupper TS. IL-15 and dermal fibroblasts induce proliferation of natural regulatory cells isolated from human skin. Blood. 2007 Jan 1;109(1):194-202.(PCH101, IHC frozen, PubMed)

Epple HJ, Loddenkemper C, Kunkel D, Tröger H, Maul J, Moos V, Berg E, Ullrich R, Schulzke JD, Stein H, Duchmann R, Zeitz M, Schneider T. Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART. Blood. 2006 Nov 1;108(9):3072-8. PCH101, IHC paraffin, PubMed)

Manigold T, Shin EC, Mizukoshi E, Mihalik K, Murthy KK, Rice CM, Piccirillo CA, Rehermann B. Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees recovered from Hepatitis C.Blood. 2006 Jun 1;107(11):4424-32. (PCH101 in chimpanzee, PubMed)

Ahmadzadeh M, Rosenberg SA. IL-2 Administration Increases CD4+CD25hiFoxp3+ Regulatory T Cells in Cancer Patients. Blood. 2006 Mar 15;107(6):2409-14. (PCH101, IC Flow, PubMed)

Hartwig UF, Nonn M, Khan S, Meyer RG, Huber C, Herr W. Depletion of alloreactive T cells via CD69: implications on antiviral, antileukemic and immunoregulatory T lymphocytes. Bone Marrow Transplant. 2006 Feb;37(3):297-305. (PCH101, IC Flow, PubMed)

Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK. Human CD4+ T Cells Express TLR5 and Its Ligand Flagellin Enhances the Suppressive Capacity and Expression of FOXP3 in CD4+CD25+ T Regulatory Cells. J Immunol. 2005 Dec 15;175(12):8051-9. (PCH101, IC Flow, PubMed)

Lim, H.W., P. Hillsamer, A.H. Banham, and C.H. Kim. 2005. Cutting Edge: Direct Suppression of B cells by CD4+CD25+ Regulatory T cells. J Immunol. 2005 Oct 1;175(7):4180-3. (PCH101,IC Flow, PubMed)

Manavalan JS, Kim-Schulze S, Scotto L, Naiyer AJ, Vlad G, Colombo PC, Marboe C, Mancini D, Cortesini R, Suciu-Foca N. Alloantigen specific CD8+CD28- FOXP3+ T suppressor cells induce ILT3+ ILT4+ tolerogenic endothelial cells, inhibiting alloreactivity. Int Immunol. 2004 Aug;16(8):1055-68.

Takahata Y, Nomura A, Takada H, Ohga S, Furuno K, Hikino S, Nakayama H, Sakaguchi S, Hara T. CD25+CD4+ T cells in human cord blood: an immunoregulatory subset with naïve phenotype and specific expression of forkhead box p3 (Foxp3) gene. Exp Hematol. 2004 Jul;32(7):622-9.

Sakaguchi S. 2003. The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery. J Clin Invest. 2003 November 1; 112(9): 1310–1312.

Hori S, Nomura T, Sakaguchi S. Control of regulatory T cell development by the transcription factor Foxp3. Science. 2003 Feb 14;299(5609):1057-61.

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