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  • Anti-Human Foxp3 Alexa Fluor 647 (Discontinued. Refer to Cat. No. 50-4777)

Anti-Human Foxp3 Alexa Fluor 647 (Discontinued. Refer to Cat. No. 50-4777)

Also known as: Forkhead Box P3, Scurfin, JM2, Treg

Clone: 236A/E7

RUO: For Research Use Only. Not for use in diagnostic procedures.

Attention: Replacement format is eFluor 660. Select Cat. No. 50-4777.

SKU# 51-4777

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Data for Anti-Human Foxp3 Alexa Fluor® 647.

Staining of normal human peripheral blood mononuclear cells with Anti-Human CD25 PE (cat. 12-0259)...View More

  • Data for Anti-Human Foxp3 Alexa Fluor® 647.
Description

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3+ cells. Other antibodies to human Foxp3 include the recently published PCH101 (cat. 72-5776) and ebio7979 (cat. 14-7979).

The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776).

Details
Host Mouse
Isotype IgG1
Reactivity Human, Rhesus
Conjugate Alexa Fluor 647
Laser Red Laser
Emit 668 nm
Excite 633 - 647 nm
Legal Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications Intracellular Staining Followed by Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
13-4777 Anti-Human Foxp3 Biotin FC, IHC RUO
50-4777 Anti-Human Foxp3 eFluor® 660 (Alexa Fluor® 647 Replacement) 633 - 647 nm 668 nm FC RUO
12-4777 Anti-Human Foxp3 PE 488 - 561 nm FC RUO
53-4777 Anti-Human Foxp3 Alexa Fluor® 488 488 nm 519 nm FC, IHC RUO
11-4777 Anti-Human Foxp3 FITC 488 nm FC RUO
17-4777 Anti-Human Foxp3 APC 633 - 647 nm 660 nm FC RUO
48-4777 Anti-Human Foxp3 eFluor® 450 405 nm FC RUO
14-4777 Anti-Human Foxp3 Purified IHC, WB RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-5521 Foxp3 Fixation/Permeabilization Concentrate and Diluent FC GPR
00-5523 Foxp3 / Transcription Factor Staining Buffer Set FC GPR
11-0049 Anti-Human CD4 FITC 488 nm 518 nm FC RUO
12-0259 Anti-Human CD25 PE 488 - 561 nm 575 nm FC RUO
51-4714 Mouse IgG1 K Isotype Control Alexa Fluor® 647 (Discontined. Refer to Cat. No. 50-4714) 633 - 647 nm 668 nm FC, ICC, IHC RUO
References

References: Sakaguchi S. 2003. The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery. J Clin Invest. 112(9):1310-2.

Hori S, Nomura T, Sakaguchi S. 2003. Control of regulatory T cell development by the transcription factor Foxp3. Science. 299(5609):1057-61.

Takahata Y, Nomura A, Takada H, Ohga S, Furuno K, Hikino S, Nakayama H, Sakaguchi S, Hara T. 2004. CD25+CD4+ T cells in human cord blood: an immunoregulatory subset with naive phenotype and specific expression of forkhead box p3 (Foxp3) gene. Exp Hematol. 32(7):622-9.

Alvaro T, Lejeune M, Salvado MT, Bosch R, Garcia JF, Jaen J, Banham AH, Roncador G, Montalban C, Piris MA. 2005. Outcome in Hodgkin's lymphoma can be predicted from the presence of accompanying cytotoxic and regulatory T cells. Clin Cancer Res. 11(4):1467-73. [IHC paraffin using 236A/E7, PubMed]

Lim, H.W., P. Hillsamer, A.H. Banham, and C.H. Kim. 2005. Cutting Edge: Direct Suppression of B cells by CD4+CD25+ Regulatory T cells. J. Immunol. 175: 4180-4183. (236A/E7, IH(frozen), PCH101, IC (flow), PubMed)

Roncador, G., P.J. Brown, L. Maestre, S. Hue, J.L. Martinez-Torrecuadrada, K.L. Ling, S. Pratap, C. Toms, B.C. Fox, V. Cerundolo, F. Powrie, and A.H. Banham. 2005. Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level. Eur J Immunol. 35: 1681-91. [236A/E7, IC(flow), IH(paraffin), PubMed]

Roncador G, Garcia JF, Garcia JF, Maestre L, Lucas E, Menarguez J, Ohshima K, Nakamura S, Banham AH, Piris MA. 2005. FOXP3, a selective marker for a subset of adult T-cell leukaemia/lymphoma. Leukemia [Epub ahead of print] [IHC paraffin sections using 236A/E7, PubMed]

Wolf D, Wolf AM, Rumpold H, Fiegl H, Zeimet AG, Muller-Holzner E, Deibl M, Gastl G, Gunsilius E, Marth C.The expression of the regulatory T cell-specific forkhead box transcription factor FoxP3 is associated with poor prognosis in ovarian cancer. Clin Cancer Res. 2005 Dec 1;11(23):8326-31. [IHC paraffin using 236A/E7, PubMed]

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