Anti-Human Foxp3 Alexa Fluor® 532

Also known as: Forkhead Box P3, Scurfin, JM2

Clone: PCH101

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 58-4776

Cat. No.	Size
58-4776-41	25 tests
58-4776-42	100 tests
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Surface staining of normal human peripheral blood cells with Anti-Human CD25 APC (cat. 17-0259)...View More

Description

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3⁺ cells. Please contact tech@ebioscience.com or 888.810.6168 for any additional assistance.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in ‘scurfy’ (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259).

Details

Host	Rat
Isotype	IgG2a, kappa
Reactivity	Human
Conjugate	Alexa Fluor 532
Laser	Green Laser
Emit	561 nm
Excite	532 nm
Legal	Alexa Fluor® is a registered trademark of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Intracellular Staining Followed by Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
14-4776	Anti-Human Foxp3 Purified			IHC, WB	RUO
48-4776	Anti-Human Foxp3 eFluor® 450	405 nm	450 nm	FC	RUO
35-4776	Anti-Human Foxp3 PE-Cyanine5.5	488 - 567 nm	690 nm	FC	RUO
25-4776	Anti-Human Foxp3 PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
11-4776	Anti-Human Foxp3 FITC	488 nm	518 nm	FC, IHC	RUO
13-4776	Anti-Human Foxp3 Biotin			FC, IHC, WB	RUO
12-4776	Anti-Human Foxp3 PE	488 - 561 nm		FC	RUO
56-4776	Anti-Human Foxp3 Alexa Fluor® 700	633 - 647 nm	723 nm	FC	RUO
17-4776	Anti-Human Foxp3 APC	633 - 647 nm	660 nm	FC	RUO
53-4776	Anti-Human Foxp3 Alexa Fluor® 488	488 nm	519 nm	FC	RUO
45-4776	Anti-Human Foxp3 PerCP-Cyanine5.5	488 nm		FC	RUO
50-4776	Anti-Human Foxp3 eFluor® 660 (Alexa Fluor® 647 Replacement)	633 nm	668 nm	FC	RUO
15-4776	Anti-Human Foxp3 PE-Cyanine5	488 - 561 nm		FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-5523	Foxp3 / Transcription Factor Staining Buffer Set			FC	GPR
17-0259	Anti-Human CD25 APC	633 - 647 nm	660 nm	FC	RUO
48-0047	Anti-Human CD4 eFluor® 450	405 nm		FC	RUO
12-9882	Anti-Human GARP PE	488 - 561 nm		FC	RUO
58-4321	Rat IgG2a K Isotype Control Alexa Fluor® 532	532 nm	561 nm	FC	RUO

References

References: Bell MP, Svingen PA, Rahman MK, Xiong Y, Faubion WA Jr. FOXP3 regulates TLR10 expression in human T regulatory cells. J Immunol. 2007 Aug 1;179(3):1893-900. (PCH101, ChIP assay, PubMed)

Clark RA, Kupper TS. IL-15 and dermal fibroblasts induce proliferation of natural regulatory cells isolated from human skin. Blood. 2007 Jan 1;109(1):194-202.(PCH101, IHC frozen, PubMed)

Epple HJ, Loddenkemper C, Kunkel D, Tröger H, Maul J, Moos V, Berg E, Ullrich R, Schulzke JD, Stein H, Duchmann R, Zeitz M, Schneider T. Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART. Blood. 2006 Nov 1;108(9):3072-8. PCH101, IHC paraffin, PubMed)

Manigold T, Shin EC, Mizukoshi E, Mihalik K, Murthy KK, Rice CM, Piccirillo CA, Rehermann B. Foxp3+CD4+CD25+ T cells control virus-specific memory T cells in chimpanzees recovered from Hepatitis C.Blood. 2006 Jun 1;107(11):4424-32. (PCH101 in chimpanzee, PubMed)

Ahmadzadeh M, Rosenberg SA. IL-2 Administration Increases CD4+CD25hiFoxp3+ Regulatory T Cells in Cancer Patients. Blood. 2006 Mar 15;107(6):2409-14. (PCH101, IC Flow, PubMed)

Hartwig UF, Nonn M, Khan S, Meyer RG, Huber C, Herr W. Depletion of alloreactive T cells via CD69: implications on antiviral, antileukemic and immunoregulatory T lymphocytes. Bone Marrow Transplant. 2006 Feb;37(3):297-305. (PCH101, IC Flow, PubMed)

Crellin NK, Garcia RV, Hadisfar O, Allan SE, Steiner TS, Levings MK. Human CD4+ T Cells Express TLR5 and Its Ligand Flagellin Enhances the Suppressive Capacity and Expression of FOXP3 in CD4+CD25+ T Regulatory Cells. J Immunol. 2005 Dec 15;175(12):8051-9. (PCH101, IC Flow, PubMed)

Lim, H.W., P. Hillsamer, A.H. Banham, and C.H. Kim. 2005. Cutting Edge: Direct Suppression of B cells by CD4+CD25+ Regulatory T cells. J Immunol. 2005 Oct 1;175(7):4180-3. (PCH101,IC Flow, PubMed)

Manavalan JS, Kim-Schulze S, Scotto L, Naiyer AJ, Vlad G, Colombo PC, Marboe C, Mancini D, Cortesini R, Suciu-Foca N. Alloantigen specific CD8+CD28- FOXP3+ T suppressor cells induce ILT3+ ILT4+ tolerogenic endothelial cells, inhibiting alloreactivity. Int Immunol. 2004 Aug;16(8):1055-68.

Takahata Y, Nomura A, Takada H, Ohga S, Furuno K, Hikino S, Nakayama H, Sakaguchi S, Hara T. CD25+CD4+ T cells in human cord blood: an immunoregulatory subset with naïve phenotype and specific expression of forkhead box p3 (Foxp3) gene. Exp Hematol. 2004 Jul;32(7):622-9.

Sakaguchi S. 2003. The origin of FOXP3-expressing CD4+ regulatory T cells: thymus or periphery. J Clin Invest. 2003 November 1; 112(9): 1310–1312.

Hori S, Nomura T, Sakaguchi S. Control of regulatory T cell development by the transcription factor Foxp3. Science. 2003 Feb 14;299(5609):1057-61.

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Antigens	Area of Biology	Cell Type	Formats
Foxp3	Adaptive Immunity Transcription Factors	T Cells Treg Cells	Alexa Fluor 532