FMC7 eFluor® 450

Description: The FMC7 monoclonal antibody recognizes a membrane glycoprotein that is expressed on mature human B lymphocytes. FMC7 recognizes a conformational epitope on the CD20 molecule that is exposed on a subset of mature B lymphocytes. The expression of this epitope has been shown to be extremely sensitive to levels of membrane cholesterol.

References: Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A6), 3rd Edition published by the National Committee for Clinical Laboratory Standards.

Brooks, DA, et al. Human lymphocyte markers defined by antibodies derived from somatic cell hybrids. IV. A monoclonal antibody reacting specifically with a subpopulation of human B lymphocytes. Journal of Immunology. 1981;126:1373-1377.

Serke, S, et al. Monoclonal antibody FMC7 detects a conformational epitope on the CD20 molecule: evidence from phenotyping after rituxan therapy and transfectant cell analyses.Cytometry. 2001;46:98-104.

Polyak, MJ, et al. A cholesterol-dependent CD20 epitope detected by the FMC7 antibody. Leukemia. 2003;17:1384-1389.