Description: The human FcγR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcγR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcγ receptors have been identified: FcγRI (CD64), FcγRII (CD32), FcγRIII (CD16), and FcγRIV, and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcγR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcγR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcγRs.
