Human E-selectin FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human sE-selectin in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit PE (cat. BMS8421FF), this kit can be used to detect sE-selectin alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A9.

Endothelial Leukocyte Adhesion Molecule-1 belongs to the selectin family of adhesion molecules. Together with L-selectin and P-selectin, E-selectin mediates the initial interactions of leukocytes and platelets with endothelial cells.

Molecular structure: The extracellular part of all selectins consists of an aminoterminal c-type lectin domain which specifically binds to carbohydrate ligands. This is followed by an EGF-like domain, and, in the case of E-selectin, by 6 short consensus repeats. The transmembrane portion of the molecule is followed by a short cytoplasmic tail.

Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. Together with P-selectin, E-selectin is expressed on cytokine-activated endothelial cells, and contributes to the adhesion of still resting leukocytes to the endothelium. This initial binding event is an essential prerequisite for the activation of the immune cells via different inflammatory mediators. In contrast to P-selectin, E-selectin is maximally expressed 2-4 hours after cell activation. Within the next 24-48 hours E-selectin is again eliminated from the cytoplasmic membrane by shedding into the circulation.

Citations: Wong,C.K.; Ho,A.W.; Tong,P.C.; Yeung,C.Y.; Chan,J.C.; Kong,A.P.; Lam,C.W.. Aberrant expression of soluble co-stimulatory molecules and adhesion molecules in type 2 diabetic patients with nephropathy. J Clin Immunol 2008;28:36-43. (Link)

Michlmayr A.,S.Baumann,M.Zellner,C.Burghuber,P.Schuch,C.Wenzel,R.Bartsch,U.Pluschnig,I.Rech-Weichselbraun,G.Steger,R.Jakesz,M.Gnant and T.Bachleitner-Hofmann,M.Bergmann,R.Oehler. Chemotherapy induces inflammatory response in breast cancer patients. Keystonesymposia, Inflammation, Microenvironment and Cancer 2008;

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