Anti-Human Cytokeratin 8 FITC

Description: This LP3K monoclonal antibody reacts with human cytokeratin 8 (K8), a 55-kDa member of the family of intermediate filament proteins. Cytokeratin 8 is a type II (or basic) keratin that is expressed in epithelial and carcinoma cells. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. More specifically, studies have demonstrated the involvement of cytokeratin 8 in protection against apoptosis, stress, or injury, as well as regulation of the cell cycle. This keratin is frequently co-expressed with cytokeratin 18, a type I (or acidic) keratin as a heterodimer. Although detected primarily in the cytoplasm of normal healthy cells, cytokeratin 8 has been found to localize to the plasma membrane in some tumor cells. Finally, cytokeratin 8 is phosphorylated on serine 73 in dividing cells.

References: Moll R, Divo M, Langbein L. The human keratins: biology and pathology. Histochem Cell Biol. 2008 Jun;129(6):705-33. Review.

Gires O, Andratschke M, Schmitt B, Mack B, Schaffrik M. Cytokeratin 8 associates with the external leaflet of plasma membranes in tumour cells. Biochem Biophys Res Commun. 2005 Mar 25;328(4):1154-62.

Waseem A, Karsten U, Leigh IM, Purkis P, Waseem NH, Lane EB. Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability. Biochemistry. 2004 Feb 10;43(5):1283-95. (LP3K, WB)