Human Chemokine 6plex FlowCytomix Multiplex

Description: This Human Chemokine 6plex FlowCytomix Multiplex is designed for the measurement of human G-CSF, IL-8, MCP-1, MIG, MIP-1alpha and MIP-1beta in an immunoassay analyzed on a flow cytometer.

Chemokines represent a superfamily of small secreted proteins that function as intercellular messengers to control migration and activation of leukocytes involved in inflammatory reactions and immunity. Furthermore, chemokines are important mediators of many pathologies such as allergic responses, chronic inflammatory and autoimmune diseases, tumor growth and hematopoietic development.

Interleukin-8/Neutrophil-Activating Peptide-1 selectively stimulates the ability of neutrophils and T-lymphocytes to invade injured or inflamed tissue.

The CC-chemokine monocyte chemoattractant protein 1 (MCP-1) is mainly expressed by macrophages in response to a wide range of cytokines such as IL-6, TNF-alpha and IL-1beta, but can, upon stimulation, also be produced by a variety of cells and tissues, such as fibroblasts, endothelial cells or certain tumor cells.

MIG (monokine induced by interferon gamma) belongs to the subfamily of the CXC chemokines. The main function of this soluble protein is the recruitment of leukocytes to sites of infection and inflammation.
MIG binds to a receptor which is selectively expressed in activated T lymphocytes and therefore is a critical mediator of T-lymphocyte migration in T-cell dependent immune responses.

Granulocyte colony stimulating factor (G-CSF) is a naturally occurring cytokine that stimulates the production and antibacterial function of neutrophils and monocytes. The pleotropic cytokine is produced by activated monocytes, macrophages, endothelial cells, fibroblasts, astrocytes, osteoblasts and bone marrow cells. G-CSF has been shown to have specific effects on the proliferation, differentiation and activation of hematopoietic cells. G-CSF is also expressed by various transformed cells such as carcinoma cells and myeloblastic leukaemia cells.

Macrophage inflammatory protein 1 alpha (MIP-1alpha) and beta (MIP-1beta) belong to the family of cysteine-cysteine (cc) chemokines. The chemokines self-associate to form high molecular mass aggregates while the monomers are low mass polypeptides. Both MIP-1alpha and MIP-1beta are not only chemoattractants but also coactivators of macrophages acting in concert with IFN-gamma as type 1 cytokines.
MIP-1alpha was shown to stimulate strong antigen specific responses, while MIP-1beta promotes antibody responses.

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