Anti-Human CD86 (B7-2) Alexa Fluor® 488

Also known as: B72, B70

Clone: IT2.2

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0869

Cat. No.	Size
53-0869-41	25 tests
53-0869-42	100 tests
Please inquire about custom configurations or bulk packaging.

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Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control Alexa Fluor® 488...View More

Description

Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.

Details

Host	Mouse
Isotype	IgG2b, kappa
Reactivity	Human
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

For complete product information, please download the TDS or IFU document.

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
13-0869	Anti-Human CD86 (B7-2) Biotin			FC	RUO
14-0869	Anti-Human CD86 (B7-2) Purified			FC, FA	RUO
15-0869	Anti-Human CD86 (B7-2) PE-Cy5	488 - 561 nm		FC	RUO
12-0869	Anti-Human CD86 (B7-2) PE	488 - 561 nm	575 nm	FC	RUO
46-0869	Anti-Human CD86 (B7-2) PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
16-0869	Anti-Human CD86 (B7-2) Functional Grade Purified			FC, FA	RUO

References

References: Kapsogeorgou EK, Moutsopoulos HM, Manoussakis MN. Functional expression of a costimulatory B7.2 (CD86) protein on human salivary gland epithelial cells that interacts with the CD28 receptor, but has reduced binding to CTLA4. J Immunol. 2001 Mar 1;166(5):3107-13. (IT2.2, FA, PubMed)

Kishimoto T, von dem Borne AEG, et al. eds. Leucocyte Typing VI: White Cell Differentiation Antigens. 1998. Garland Publishing Inc. London.

Jeannin P, Delneste Y, Lecoanet-Henchoz S, Gauchat JF, Ellis J, Bonnefoy JY. CD86 (B7-2) on human B cells. A functional role in proliferation and selective differentiation into IgE- and IgG4-producing cells. J Biol Chem. 1997 Jun 20;272(25):15613-9. (IT2.2, FA, PubMed)

Azuma M, Ito D, Yagita H, Okumura K, Phillips JH, Lanier LL, Somoza C. B70 antigen is a second ligand for CTLA-4 and CD28. Nature. 1993 Nov 4;366(6450):76-9. (IT2.2, FC, IP, PubMed)

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD86 (B7-2)	Innate Immunity	Dendritic Cells	Alexa Fluor 488