Anti-Human CD85h (ILT1) Functional Grade Purified

Description: The monoclonal antibody 135.4 recognizes ILT1, also known as CD85h, LIR7 and LILRA2. ILT1 is a member of the large family of ILT molecules (Immunologulin-like Transcripts) also known as Leukocyte Ig-like Receptors (LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). ILT1 contains a short cytoplasmic domain. Through the cytoplasmic domain ILT1 associates with the Fc receptor gamma chain but not DAP12 resulting in a stimulatory signal. ILT1 may act as a receptor for MHC Class I antigens. Expression of ILT1 is mainly found on monocytes, macrophages, DC and weakly on granulocytes. ILT1 is thought to play a role in the TH1/Th2 balance as well as TLR signaling.

The monoclonal antibody 135.4 has been reported to have activating properties upon crosslinking which result in calcium influx.

References: Mamegano K, Kuroki K, Miyashita R, Kusaoi M, Kobayashi S, Matsuta K, Maenaka K, Colonna M, Ozaki S, Hashimoto H, Takasaki Y, Tokunaga K, Tsuchiya N. Association of LILRA2 (ILT1, LIR7) splice site polymorphism with systemic lupus erythematosus and microscopic polyangiitis. Genes Immun. 2008 Feb 14;1-10 (135.4, FC, PubMed)

Penna G, Roncari A, Amuchastegui S, Daniel KC, Berti E, Colonna M, Adorini L. Expression of the inhibitory receptor ILT3 on dendritic cells is dispensable for induction of CD4+Foxp3+ regulatory T cells by 1,25-dihydroxyvitamin D3. Blood. 2005 Nov 15;106(10):3490-7. (135.4, FA,PubMed)

Nakajima H, Samaridis J, Angman L, Colonna M.Human myeloid cells express an activating ILT receptor (ILT1) that associates with Fc receptor gamma-chain.J Immunol. 1999 Jan 1;162(1):5-8. (135.4, FC, IP, FA PubMed)