Anti-Human CD85g (ILT7) APC

Description: The eBio17G10.2 monoclonal antibody reacts with human immunoglobulin-like transcript 7 (ILT7), also known as CD85g. ILT7 is part of the leukocyte immunoglobulin-like receptor (LIR) gene family and associates with FcεR1γ to form a functional complex which transduces ITAM-mediated signals that negatively modulates TLR-induced type I IFN production by human plasmacytoid dendritic cells. ILT7 is expressed exclusively on plasmacytoid dendritic cells and is absent in monocyte derived DCs. Treatment with IL-3 or CpG causes a decrease in expression.

Double staining of human PBMCs with eBio17G10.2 and anti-pDC marker BDCA2 yields virtually complete co-staining. Cross linking of ILT7 with eBio17G10.2 antibody causes reduced transcription of IFN-α1, IFN-α2, IFN-α4 and IFN-β.

References: Cao, W., et al. 2006. Plasmacytoid dendritic cell–specific receptor ILT7–FcRI inhibits Toll-like receptor–induced interferon production. J. Exp. Med. 203: 1399-1405.

Ju XS, Hacker C, Scherer B, Redecke V, Berger T, Schuler G, Wagner H, Lipford GB, Zenke M. Immunoglobulin-like transcripts ILT2, ILT3 and ILT7 are expressed by human dendritic cells and down-regulated following activation. Gene. 2004 Apr 28;331:159-64.

Rissoan MC, Duhen T, Bridon JM, Bendriss-Vermare N, Peronne C, de Saint Vis B, Briere F, Bates EE. Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells. Blood. 2002 Nov 1;100(9):3295-303.