Anti-Human CD85d (ILT4) Functional Grade Purified

Description: The monoclonal antibody 42D1 recognizes the CD85d protein also known as ILT4, LIR-2. It is a member of the ILT (immunoglobulin-like transcript)/ LIR (leukocyte Ig-like receptor), MIR (monocytes Ig-like receptor) family. CD85d is a 110 kDa single transmembrane receptor with 3 Ig-like domains and a long cytoplasmic domain containing ITIMs (immunoreceptor tyrosine-based inhibitory motifs). Expression is confined to monocytes, macrophages and dendritic cells. The ligand for CD85d is non-classical class I HLA-G, -B and G1. Upon ligand binding, tyrosine phosphatase SHP-1 interacts with the ITIM. Interaction of HLA-G with ILT4/CD85d is thought to contribute to tolerance during fetal implantation. Additionally, it is the levels of ILT3 and ILT4 on DCs that suggests a role in organ allograft rejection.

Crosslinking of the receptor with the monoclonal antibody 42D1 inhibits calcium flux as well as enhancing HLA-G tetramer staining and restoring the stimulating activity of tolerogenic DC.

References: Colonna M, Samaridis J, Cella M, Angman L, Allen RL, O'Callaghan CA, Dunbar R, Ogg GS, Cerundolo V, Rolink A.Human myelomonocytic cells express an inhibitory receptor for classical and nonclassical MHC class I molecules.J Immunol. 1998 Apr 1;160(7):3096-100.

Allan DS, Colonna M, Lanier LL, Churakova TD, Abrams JS, Ellis SA, McMichael AJ, Braud VM. Tetrameric complexes of human histocompatibility leukocyte antigen (HLA)-G bind to peripheral blood myelomonocytic cells.J Exp Med. 1999 Apr 5;189(7):1149-56.

Colonna M, Nakajima H, Navarro F, López-Botet M.A novel family of Ig-like receptors for HLA class I molecules that modulate function of lymphoid and myeloid cells.J Leukoc Biol. 1999 Sep;66(3):375-81

Manavalan JS, Rossi PC, Vlad G, Piazza F, Yarilina A, Cortesini R, Mancini D, Suciu-Foca N. High expression of ILT3 and ILT4 is a general feature of tolerogenic dendritic cells.Transpl Immunol. 2003 Jul-Sep;11(3-4):245-58. (42D1, FA,FC, PubMed)