Anti-Human CD58 (LFA-3) Functional Grade Purified

Description: The TS2/9 antibody recognizes human CD58, which is also known as Lymphocyte Associated Antigen-3 (LFA-3). CD58 exists both as a transmembrane protein as well as a GPI-linked extracellular protein. It has been shown that cells can express both forms concurrently. In addition, soluble CD58 has also been observed in serum, urine and synoval fluid of rheumatoid arthritis patients. CD58 is found on non-hematopoietic and most hematopoietic cells. Binding to its ligand CD2, which is present on T cells, initiates a signaling pathway which includes phosphorylation and results in release of cytokines. CD58 has been shown to be important in adhesion to various cell types, T cell activation, cytokine production and cytoxicity.
The TS2/9 antibody has been reported to induce signaling events upon crosslinking. The epitope is in domain 1 of the immunoglobulin region.

References: Ariel O, Levi Y, Hollander N. Signal Transduction by CD58: The transmembrane isoform transmits signals outside lipid rafts independently of the GPI-anchored isoform. Cellular Signaling. 2009:21:1100-1108. (TS/9, functional crosslinking, PubMed)

Osborn L, Day ES, Miller GT, Karpusas M, Tizard R, Meuer SC, Hochman PS. Amino acid residues required for binding of Lymphocyte Function-associated Antigen 3 (CD58) to its Counter-Receptor CD2. J. Exp. Med. January 1995:181:429-434.

Diaz-Sanchez, Chegini S, Zhang K, Saxon A. CD58 (LFA-3) stimulation provides a signal for human isotype switching and IgE production distinct from CD40. J. Immunol. 1994:153:10-20. (TS/9, FA, PubMed)

Hahn WC, Burakoff SJ, Bierer BE. Signal Transduction Pathways involved in T-cell receptor-induced regulation of CD2 avidity for CD58. J. Immunol. 1993:150:2607-2619. (TS/9, FC, PubMed)

Le PT, Vollger LW, Haynes BF, Singer KH. Ligand binding to the LFA-3 cell adhesion molecule induces IL-1 production by human thymic epithelial cells. J. Immunol. 1990:144:4541-4547. (TS/9, mAb characterization, PubMed)

Sanchez-Madrid F, Krensky AM, Ware CF, Robbins E, Strominger JL, Burakoff SJ, Springer TA. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci USA. December 1982:79:7489-7493.