Human ICAM-1 FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human sICAM-1 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit PE (cat. BMS8421FF), this kit can be used to detect sICAM-1 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A11.

Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-beta-complex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors. This family consists of the leukocyte adhesion glycoproteins LFA-1 which mediates lymphocyte adhesion, Mac-1 which mediates granulocyte adhesion and p150,95.

ICAM-1 is a single-chain glycoprotein with a polypeptide core that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B-cells and dendritic cells in tonsils, lymph nodes and Peyer's patches. ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.

The role of ICAM-1 as a disease marker has been demonstrated for a number of different indications and pathological situations.

Citations: Wong,C.K.; Ho,A.W.; Tong,P.C.; Yeung,C.Y.; Chan,J.C.; Kong,A.P.; Lam,C.W.. Aberrant expression of soluble co-stimulatory molecules and adhesion molecules in type 2 diabetic patients with nephropathy. J Clin Immunol 2008;28:36-43. (Link)

Michlmayr A.,S.Baumann,M.Zellner,C.Burghuber,P.Schuch,C.Wenzel,R.Bartsch,U.Pluschnig,I.Rech-Weichselbraun,G.Steger,R.Jakesz,M.Gnant and T.Bachleitner-Hofmann,M.Bergmann,R.Oehler. Chemotherapy induces inflammatory response in breast cancer patients. Keystonesymposia, Inflammation, Microenvironment and Cancer 2008;

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Dustin,M.L.; Rothlein,R.; Bhan,A.K.; Dinarello,C.A.; Springer,T.A.. Induction by IL 1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J.Immunol. 1986;137:245-254. (Link)

Lampeter,E.R.; Kishimoto,T.K.; Rothlein,R.; Mainolfi,E.A.; Bertrams,J.; Kolb,H.; Martin,S.. Elevated levels of circulating adhesion molecules in IDDM patients and in subjects at risk for IDDM. Diabetes 1992;41:1668-1671. (Link)

Vejlsgaard,G.L.; Ralfkiaer,E.; Avnstorp,C.; Czajkowski,M.; Marlin,S.D.; Rothlein,R.. Kinetics and characterization of intercellular adhesion molecule-1 (ICAM-1) expression on keratinocytes in various inflammatory skin lesions and malignant cutaneous lymphomas. J.Am.Acad.Dermatol. 1989;20:782-790. (Link)

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Most,J.; Zangerle,R.; Herold,M.; Fuchs,D.; Wachter,H.; Fritsch,P.; Dierich,M.P.. Elevated concentrations of circulating intercellular adhesion molecule 1 (ICAM-1) in HIV-1 infection. J.Acquir.Immune Defic.Syndr. 1993;6:221-226. (Link)

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Harning,R.; Mainolfi,E.; Bystryn,J.C.; Henn,M.; Merluzzi,V.J.; Rothlein,R.. Serum levels of circulating intercellular adhesion molecule 1 in human malignant melanoma. Cancer Res. 1991;51:5003-5005. (Link)

Adams,D.H.; Hubscher,S.G.; Shaw,J.; Rothlein,R.; Neuberger,J.M.. Intercellular adhesion molecule 1 on liver allografts during rejection. Lancet 1989;2:1122-1125. (Link)

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Marlin,S.D.; Springer,T.A.. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell 1987;51:813-819. (Link)

Adams,D.H.; Mainolfi,E.; Elias,E.; Neuberger,J.M.; Rothlein,R.. Detection of circulating intercellular adhesion molecule-1 after liver transplantation--evidence of local release within the liver during graft rejection. Transplantation 1993;55:83-87. (Link)

Rothlein,R.; Czajkowski,M.; O'Neill,M.M.; Marlin,S.D.; Mainolfi,E.; Merluzzi,V.J.. Induction of intercellular adhesion molecule 1 on primary and continuous cell lines by pro-inflammatory cytokines. Regulation by pharmacologic agents and neutralizing antibodies. J.Immunol. 1988;141:1665-1669. (Link)

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