Human ICAM-3 FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human sICAM-3 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit PE (cat. BMS8421FF), this kit can be used to detect sICAM-3 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A7.

ICAM-3 is a heavily glycosylated, integral membrane protein with five immunoglobulin-like domains. It shares high homology to ICAM-1 and ICAM-2 in the extracellular region. In contrast to ICAM-1 and ICAM-2, ICAM-3 is absent on endothelia. ICAM-3 is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. The finding that adhesion of resting T lymphocytes to LFA-1 occurs primarily via ICAM-3 combined with the fact that ICAM-3 is much better expressed than other LFA-1 ligands on monocytes and resting lymphocytes implies an important role for ICAM-3 in the initiation of immune responses.

The initial interaction of ICAM-3 with LFA-1 might increase LFA-1-mediated cell binding to ICAM-1.

Furthermore, ICAM-3 expression has been shown for dendritic epidermal Langerhans cells, whereas it is absent on other dendritic cells from different lymphoid organs. Thus potential function of ICAM-3 at the initiation phase of LC-leukocyte interactions taking place during skin localized immune reactions can be postulated.

Citations: Wong,C.K.; Ho,A.W.; Tong,P.C.; Yeung,C.Y.; Chan,J.C.; Kong,A.P.; Lam,C.W.. Aberrant expression of soluble co-stimulatory molecules and adhesion molecules in type 2 diabetic patients with nephropathy. J Clin Immunol 2008;28:36-43. (Link)

Michlmayr A.,S.Baumann,M.Zellner,C.Burghuber,P.Schuch,C.Wenzel,R.Bartsch,U.Pluschnig,I.Rech-Weichselbraun,G.Steger,R.Jakesz,M.Gnant and T.Bachleitner-Hofmann,M.Bergmann,R.Oehler. Chemotherapy induces inflammatory response in breast cancer patients. Keystonesymposia, Inflammation, Microenvironment and Cancer 2008;

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