Anti-Human CD47 Purified

Description: The monoclonal antibody B6H12 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small alternatively spliced cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins CD51/CD61 and CD41/CD61. Furthermore this interaction can mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. CD47 is the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Recently stimulation of CD47 by monoclonal antibody has been shown to induce CD4+CD25- suppressive activity also increasing expression of Foxp3. Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.

This antibody has been reported to have neutralizing activity.

References: Grimbert P, Bouguermouh S, Baba N, Nakajima T, Allakhverdi Z, Braun D, Saito H, Rubio M, Delespesse G, Sarfati M. Thrombospondin/CD47 interaction: a pathway to generate regulatory T cells from human CD4+ CD25- T cells in response to inflammation.J Immunol. 2006 Sep 15;177(6):3534-41. (B6H12, FA)

Lagadec P, Dejoux O, Ticchioni M, Cottrez F, Johansen M, Brown EJ, Bernard A. Involvement of a CD47-dependent pathway in platelet adhesion on inflamed vascular endothelium under flow.Blood. 2003 Jun 15;101(12):4836-43.(B6H12, FA)

Brown E, Hooper L, Ho T, Gresham H. Integrin-associated protein: a 50-kD plasma membrane antigen physically and functionally associated with integrins.J Cell Biol. 1990 Dec;111(6 Pt 1):2785-94. (B6H12, WB)

Gresham HD, Goodwin JL, Allen PM, Anderson DC, Brown EJ.A novel member of the integrin receptor family mediates Arg-Gly-Asp-stimulated neutrophil phagocytosis.J Cell Biol. 1989 May;108(5):1935-43.