Description: This three-color cocktail is designed to identify T cells and B cells from peripheral blood samples. It can be used alone, or in combination with other antibodies to further characterize these lymphocyte populations. The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as the Leukocyte Common Antigen (LCA). CD45 is expressed by all leukocytes and is not expressed on circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in the activation of lymphocytes. The SK7 monoclonal antibody reacts with human CD3 eplison (CD3ε, CD3e), a 20 kDa subunit of the CD3/TCR complex. The CD3 complex, including the ε, γ and δ subunits, is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development except for terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19 forms a complex with CD21, CD81, Leu13, and MHC class II that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.
This cocktail contains the following antibodies:
Anti-Human CD45 (2D1) FITC
Anti-Human CD3 (SK7) PerCP-eFluor® 710
Anti-Human CD19 (SJ25C1) APC