Human CD44var (v6) FlowCytomix Simplex

Description: This FlowCytomix Simplex Kit is designed for the measurement of Human CD44var in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect CD44var alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population A7.

Alternative splicing of the mRNA gives rise to the different isoforms, containing inserts of varying sizes in the extracellular domain of the molecule (exons v2-v10). All CD44 isoforms are variably glycosylated. In contrast to standard CD44 (CD44std) which is almost ubiquitously expressed, the variety of CD44 isoforms (CD44var) have a much more restricted distribution, e.g., on activated lymphocytes and macrophages (exon v6).

The sCD44var(v6) ELISA detects all circulating CD44 isoforms comprising the sCD44var(v6) sequences.

Determination of sCD44var(v6) will provide more detailed insight into different pathological modifications during cancer and other diseases.

Citations: Kanke,Minoru; Fujii,Masato; Kameyama,Kaori; Kanzaki,Jin; Tokumaru,Yutaka; Imanishi,Yorihisa; Tomita,Toshiki; Matsumura,Yasuhiro. Role of CD44 Variant Exon 6 in Invasion of Head and Neck Squamous Cell Carcinoma. Archives of Otolaryngology - Head and Neck Surgery 2000;126:1217-1223. (Link)

References: Humbria,A.; az-Gonzalez,F.; Campanero,M.R.; Arroyo,A.G.; Laffon,A.; Gonzalez-Amaro,R.; Sanchez-Madrid,F.. Expression of L-selectin, CD43, and CD44 in synovial fluid neutrophils from patients with inflammatory joint diseases. Evidence for a soluble form of L-selectin in synovial fluid. Arthritis Rheum. 1994;37:342-348. (Link)

Arch,R.; Wirth,K.; Hofmann,M.; Ponta,H.; Matzku,S.; Herrlich,P.; Zoller,M.. Participation in normal immune responses of a metastasis-inducing splice variant of CD44. Science 1992;257:682-685. (Link)

Hofmann,M.; Rudy,W.; Zoller,M.; Tolg,C.; Ponta,H.; Herrlich,P.; Gunthert,U.. CD44 splice variants confer metastatic behavior in rats: homologous sequences are expressed in human tumor cell lines. Cancer Res. 1991;51:5292-5297. (Link)

Heider,K.H.; Dammrich,J.; Skroch-Angel,P.; Muller-Hermelink,H.K.; Vollmers,H.P.; Herrlich,P.; Ponta,H.. Differential expression of CD44 splice variants in intestinal- and diffuse-type human gastric carcinomas and normal gastric mucosa. Cancer Res. 1993;53:4197-4203. (Link)

Stamenkovic,I.; Aruffo,A.; Amiot,M.; Seed,B.. The hematopoietic and epithelial forms of CD44 are distinct polypeptides with different adhesion potentials for hyaluronate-bearing cells. EMBO J. 1991;10:343-348. (Link)

Fujiwara,K.; Yoshino,T.; Miyake,K.; Ohara,N.; Akagi,T.. Expression of a lymphocyte adhesion molecule (CD44) in malignant lymphomas: relevance to primary site, histological subtype and clinical stage. Acta Med.Okayama 1993;47:215-222. (Link)

Moller,P.; Eichelmann,A.; Mechtersheimer,G.; Koretz,K.. Expression of beta 1-integrins, H-CAM (CD44) and LECAM-1 in primary gastro-intestinal B-cell lymphomas as compared to the adhesion receptor profile of the gut-associated lymphoid system, tonsil and peripheral lymph node. Int J.Cancer 1991;49:846-855. (Link)

Salmi,M.; Gron-Virta,K.; Sointu,P.; Grenman,R.; Kalimo,H.; Jalkanen,S.. Regulated expression of exon v6 containing isoforms of CD44 in man: downregulation during malignant transformation of tumors of squamocellular origin. J.Cell Biol. 1993;122:431-442. (Link)

Heider,K.H.; Hofmann,M.; Hors,E.; van den,Berg F.; Ponta,H.; Herrlich,P.; Pals,S.T.. A human homologue of the rat metastasis-associated variant of CD44 is expressed in colorectal carcinomas and adenomatous polyps. J.Cell Biol. 1993;120:227-233. (Link)

Gunthert,U.; Hofmann,M.; Rudy,W.; Reber,S.; Zoller,M.; Haussmann,I.; Matzku,S.; Wenzel,A.; Ponta,H.; Herrlich,P.. A new variant of glycoprotein CD44 confers metastatic potential to rat carcinoma cells. Cell 1991;65:13-24. (Link)

Kuppner,M.C.; Van,Meir E.; Gauthier,T.; Hamou,M.F.; de,Tribolet N.. Differential expression of the CD44 molecule in human brain tumours. Int.J.Cancer 1992;50:572-577. (Link)

Joensuu,H.; Ristamaki,R.; Klemi,P.J.; Jalkanen,S.. Lymphocyte homing receptor (CD44) expression is associated with poor prognosis in gastrointestinal lymphoma. Br.J.Cancer 1993;68:428-432. (Link)

Koopman,G.; Heider,K.H.; Horst,E.; Adolf,G.R.; van den,Berg F.; Ponta,H.; Herrlich,P.; Pals,S.T.. Activated human lymphocytes and aggressive non-Hodgkin's lymphomas express a homologue of the rat metastasis-associated variant of CD44. J.Exp.Med. 1993;177:897-904. (Link)

Matsumura,Y.; Tarin,D.. Significance of CD44 gene products for cancer diagnosis and disease evaluation. Lancet 1992;340:1053-1058. (Link)

Guo,M.M.; Hildreth,J.E.. HIV-induced loss of CD44 expression in monocytic cell lines. J.Immunol. 1993;151:2225-2236. (Link)

Omary,M.B.; Trowbridge,I.S.; Letarte,M.; Kagnoff,M.F.; Isacke,C.M.. Structural heterogeneity of human Pgp-1 and its relationship with p85. Immunogenetics 1988;27:460-464. (Link)