Anti-Human CD36 Alexa 647 (To Be Discontinued. Refer to Replacement Format eFluor® 660, cat. 50-0369)

Description: The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak^a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to “minimally” oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis.

References: Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F. Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07.Blood. 1993 Dec 15;82(12):3637-47. (NL07, FC, PubMed)

Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F. Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07.Cell Immunol. 1991 Oct 15;137(2):487-500. (NL07, FC, PubMed)

Gruarin P, Ulliers D, Thorne RF, Alessio M. Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb.Mol Cell Biochem. 2000 Nov;214(1-2):89-95. (NL07, PubMed)