Anti-Human CD326 (EpCAM) Alexa Fluor® 488

Also known as: Epithelial cell adhesion molecule, KSA, TROP1

Clone: MH99

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-8326

Cat. No.	Size
53-8326-41	25 tests
53-8326-42	100 tests
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Staining of A549 cells with Mouse IgG2a K Isotype Control Alexa Fluor® 488 (cat. 53-4724) (blue...View More

Description

Description: The monoclonal antibody MH99 recognizes human CD326 also known as EpCAM (Epithelial cell adhesion molecule). This 40 kDa type I transmembrane protein is involved in cell-cell interactions in a calcium-independent manner. EpCAM is expressed primarily on the basolateral surface of most epithelia. Although normal epithelia express low levels of CD326, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype. CD326 has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM.

Based on crossblocking studies, MH99 and 1B7 see overlapping epitopes.

Details

Host	Mouse
Isotype	IgG2a, kappa
Reactivity	Human
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® is a registered trademark of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
53-4724	Mouse IgG2a K Isotype Control Alexa Fluor® 488	488 nm	519 nm	FC, ICC, IHC	RUO

References

References: Rao CG, Chianese D, Doyle GV, Miller MC, Russell T, Sanders RA Jr, Terstappen LW. Expression of epithelial cell adhesion molecule in carcinoma cells present in blood and primary and metastatic tumors. Int J Oncol. 2005 Jul;27(1):49-57.

Federici MF, Kudryashov V, Saigo PE, Finstad CL, Lloyd KO. Selection of carbohydrate antigens in human epithelial ovarian cancers as targets for immunotherapy: serous and mucinous tumors exhibit distinctive patterns of expression. Int J Cancer. 1999 Apr 12;81(2):193-8. (MH99, IHC-fr, PubMed)

Litvinov SV, Bakker HA, Gourevitch MM, Velders MP, Warnaar SO. Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion. Cell Adhes Commun. 1994 Oct;2(5):417-28.

Litvinov SV, Velders MP, Bakker HA, Fleuren GJ, Warnaar SO. Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol. 1994 Apr;125(2):437-46.

Mattes MJ, Cairncross JG, Old LJ, Lloyd KO. Monoclonal antibodies to three widely distributed human cell surface antigens. Hybridoma. 1983;2(3):253-64.

View More Products

Antigens	Area of Biology	Cell Type	Formats
CD326 (EpCAM)	Adhesion & Migration	B Cells Macrophage & Monocyte Cells Embryonic Stem Cells T Cells	Alexa Fluor 488