Anti-CD324 (E-Cadherin) eFluor® 660 (Alexa Fluor® 647 Replacement)

Description: The monoclonal antibody DECMA-1 recognizes mouse, human and canine CD324 also known as E-cadherin (Epithelial cadherin) or uvomorulin. Like the other cadherin family members P and N cadherin, E-cadherin is a transmembrane glycoprotein involved in intercellular adhesion. These proteins share a common basic structure. The extracellular portions of the proteins are largely composed of repeating domains, each with two consensus Ca2+-binding motifs. The cytoplasmic domain interacts with a-, b-, and g-catenins and actinins. These catenins connect E-cadherin with the cytoskeleton.

Expression is found in most epidermal cells including melanocytes and kerotinocytes. E-cadherin is localized at the intercellular boundaries of epithelial cells in several tissues, and is thought to play a role in maintenance of tissue integrity. Loss of E-cadherin function has been implicated in the progression of a variety of cancers.

E-Cadherin protein is sensitive to trypsin treatment, so exposure to trypsin should be minimized or avoided.

The monoclonal antibody DECMA-1 has been shown to have functional activity by disrupting adhesion in human, mouse and dog cells.

References: Spencer HL, Eastham AM, Merry CL, Southgate TD, Perez-Campo F, Soncin F, Ritson S, Kemler R, Stern PL, Ward CM. E-cadherin inhibits cell surface localization of the pro-migratory 5T4 oncofetal antigen in mouse embryonic stem cells. Mol Biol Cell. 2007 Aug;18(8):2838-51. (DECMA-1, FC, WB, FA, (in mouse))

Nakagawa M, Fukata M, Yamaga M, Itoh N, Kaibuchi K. Recruitment and activation of Rac1 by the formation of E-cadherin-mediated cell-cell adhesion sites. J Cell Sci. 2001 May;114(Pt 10):1829-38. (DECMA-1, FA in canine cells, PubMed)

Lee MG, Sharrow SO, Farr AG, Singer A, Udey MC. Expression of the homotypic adhesion molecule E-cadherin by immature murine thymocytes and thymic epithelial cells. J Immunol. 1994 Jun 15;152(12):5653-9. (DECMA-1, FC in mouse cells)

Tang A, Eller MS, Hara M, Yaar M, Hirohashi S, Gilchrest BA. E-cadherin is the major mediator of human melanocyte adhesion to keratinocytes in vitro. J Cell Sci. 1994 Apr;107 ( Pt 4):983-92. (DECMA-1, FC on human cells)

Vestweber D, Kemler R. Identification of a putative cell adhesion domain of uvomorulin. EMBO J. 1985 Dec 16;4(13A):3393-8. (DECMA-1, IP, WB, FA, PubMed)