eZFluor™ Anti-Human CD3 FITC and CD8 PE Cocktail

Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to distinguish human peripheral CD3⁺ and CD8a⁺ T cells. The UCHT1 monoclonal antibody (mouse IgG1, κ) reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The RPA-T8 monoclonal antibody (mouse IgG1, κ) reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8b chain (CD8αβ) or as a homodimer (CD8αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

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