eZFluor™ Anti-Human CD3 FITC and CD4 PE Cocktail

Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to distinguish human peripheral CD3⁺ and CD4⁺ T cells. The UCHT1 monoclonal antibody (mouse IgG1, κ) reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The RPA-T4 monoclonal antibody (mouse IgG1, κ) reacts with the human CD4 molecule, a 59 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV).

References: Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.