Anti-Human CD3 Alexa Fluor® 532

Also known as: Leu-4, T3

Clone: UCHT1

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 58-0038

Cat. No.	Size
58-0038-41	25 tests
58-0038-42	100 tests
Please inquire about custom configurations or bulk packaging.

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Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (cat. 48-0199) and...View More

Description

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Details

Host	Mouse
Isotype	IgG1, kappa
Reactivity	Human
Conjugate	Alexa Fluor 532
Laser	Green Laser
Emit	561 nm
Excite	532 nm
Legal	Alexa Fluor® is a registered trademark of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
16-0038	Anti-Human CD3 Functional Grade Purified			FC, FA	RUO
56-0038	Anti-Human CD3 Alexa Fluor® 700	633 - 647 nm	723 nm	FC	RUO
14-0038	Anti-Human CD3 Purified			FC, IHC, FA	RUO
13-0038	Anti-Human CD3 Biotin			FC	RUO
17-0038	Anti-Human CD3 APC	633 - 647 nm	660 nm	FC	RUO
15-0038	Anti-Human CD3 PE-Cyanine5	488 - 561 nm		FC	RUO
12-0038	Anti-Human CD3 PE	488 - 561 nm	575 nm	FC	RUO
47-0038	Anti-Human CD3 APC-eFluor® 780	633 - 647 nm	780 nm	FC	RUO
48-0038	Anti-Human CD3 eFluor® 450	405 nm		FC	RUO
25-0038	Anti-Human CD3 PE-Cyanine7	488 - 561 nm	775 nm	FC	RUO
11-0038	Anti-Human CD3 FITC	488 nm	518 nm	FC	RUO

Related Products

Cat. No.	Name	Excite	Emit	Application	Reg.
00-4222	Flow Cytometry Staining Buffer			FC	GPR
48-0199	Anti-Human CD19 eFluor® 450	405 nm	450 nm	FC	RUO
58-4714	Mouse IgG1 K Isotype Control Alexa Fluor® 532	532 nm	561 nm	FC	RUO

References

References: Senechal B, Elain G, et al. 2007. Expansion of regulatory T cells in patients with Langerhans cell histiocytosis. PLoS Med. 14;4(8):e253. (IHC frozen, PubMed)

Baeten D, De Keyser F, et al. 2004. Tumour necrosis factor alpha independent disease mechanisms in rheumatoid arthritis: a histopathological study on the effect of infliximab on rheumatoid nodules. Ann Rheum Dis. 63(5):489-93. (IHC frozen, PubMed)

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Pollard K, Lunny D, et al. 1987. Fixation, processing, and immunochemical reagent effects on preservation of T-lymphocyte surface membrane antigens in paraffin-embedded tissue. J Histochem Cytochem. 35(11):1329-38. (IHC paraffin and frozen, PubMed)

McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

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Antigens	Area of Biology	Cell Type	Formats
CD3	Adaptive Immunity Cell Signaling	NK & NKT Cells T Cells Th17 Cells Treg Cells	Alexa Fluor 532