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Anti-Human CD3 Alexa Fluor® 488

Also known as: Leu-4, T3

Clone: OKT3

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0037

Cat. No. Size
53-0037-41 25 tests
53-0037-42 100 tests
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Data for Anti-Human CD3 Alexa Fluor® 488.

Staining of normal human peripheral blood cells with Anti-Human CD19 PE (cat. 12-0199) and staining...View More

  • Data for Anti-Human CD3 Alexa Fluor® 488.
Description

Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Details
Host Mouse
Isotype IgG2a, kappa
Reactivity Human
Conjugate Alexa Fluor 488
Laser Blue Laser
Emit 519 nm
Excite 488 nm
Legal Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
12-0037 Anti-Human CD3 PE 488 - 561 nm 578 nm FC RUO
45-0037 Anti-Human CD3 PerCP-Cyanine5.5 488 nm 695 nm FC RUO
16-0037 Anti-Human CD3 Functional Grade Purified FC, FA RUO
86-0037 Anti-Human CD3 eVolve™ 655 FC RUO
14-0037 Anti-Human CD3 Purified FC RUO
17-0037 Anti-Human CD3 APC 633 - 647 nm 660 nm FC RUO
11-0037 Anti-Human CD3 FITC 488 nm 520 nm FC RUO
46-0037 Anti-Human CD3 PerCP-eFluor® 710 488 nm 710 nm FC RUO
83-0037 Anti-Human CD3 eVolve™ 605 605 nm FC RUO
63-0037 Anti-Human CD3 Super Bright 600 405 nm 600 nm FC RUO
25-0037 Anti-Human CD3 PE-Cyanine7 488 - 561 nm 775 nm FC RUO
48-0037 Anti-Human CD3 eFluor® 450 405 nm 445 nm FC RUO
50-0037 Anti-Human CD3 eFluor® 660 633 - 647 nm 668 nm FC RUO
56-0037 Anti-Human CD3 Alexa Fluor® 700 633 - 647 nm 723 nm FC RUO
13-0037 Anti-Human CD3 Biotin FC RUO
47-0037 Anti-Human CD3 APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
53-4724 Mouse IgG2a K Isotype Control Alexa Fluor® 488 488 nm 519 nm FC, ICC, IHC RUO
12-0199 Anti-Human CD19 PE 488 - 561 nm 578 nm FC RUO
References

References: Tanaka Y, Koyanagi Y, Tanaka R, Kumazawa Y, Nishimura T, Yamamoto N. Productive and lytic infection of human CD4+ type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1. J Virol. 1997 Jan;71(1):465-70.

Hutchcroft JE, Tsai B, Bierer BE. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J Biol Chem. 1996 Jun 7;271(23):13362-70.

Adair JR, Athwal DS, Bodmer MW, Bright SM, Collins AM, Pulito VL, Rao PE, Reedman R, Rothermel AL, Xu D, et al. Humanization of the murine anti-human CD3 monoclonal antibody OKT3. Hum Antibodies Hybridomas. 1994;5(1-2):41-7.

Kung PC and Goldstein G. Complement-fixing monoclonal antibody to human T cells, and methods of preparing same. U.S. Pat. 4,361,549 dated Nov. 30, 1982.

Hoffman RA, Kung PC, Hansen WP, Goldstein G. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4914-7.