Anti-Human CD3 Alexa Fluor® 488

Also known as: Leu-4, T3

Clone: OKT3

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 53-0037

Cat. No.	Size
53-0037-41	25 tests
53-0037-42	100 tests

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Description

Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Details

Host	Mouse
Isotype	IgG2a, kappa
Reactivity	Human
Conjugate	Alexa Fluor 488
Laser	Blue Laser
Emit	519 nm
Excite	488 nm
Legal	Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Reported Applications	Flow Cytometric Analysis

Documentation

For complete product information, please download the TDS or IFU document.

TDS Link	Download TDS

Additional Formats

Cat. No.	Name	Excite	Emit	Application	Reg.
48-0037	Anti-Human CD3 eFluor® 450	405 nm	450 nm	FC	RUO
45-0037	Anti-Human CD3 PerCP-Cy5.5	488 nm	690 nm	FC	RUO
56-0037	Anti-Human CD3 Alexa Fluor® 700	633 nm	723 nm	FC	RUO
17-0037	Anti-Human CD3 APC	633 nm	660 nm	FC	RUO
95-0037	Anti-Human CD3 eFluor® 650NC	350 - 635 nm	650 nm	FC	RUO
93-0037	Anti-Human CD3 eFluor® 605NC	350 - 590 nm	605 nm	FC	RUO
51-0037	Anti-Human CD3 Alexa Fluor® 647	633 nm	668 nm	FC	RUO
12-0037	Anti-Human CD3 PE	488 nm	575 nm	FC	RUO
16-0037	Anti-Human CD3 Functional Grade Purified			FC, FA	RUO
46-0037	Anti-Human CD3 PerCP-eFluor® 710	488 nm	710 nm	FC	RUO
11-0037	Anti-Human CD3 FITC	488 nm	518 nm	FC	RUO
13-0037	Anti-Human CD3 Biotin			FC	RUO
14-0037	Anti-Human CD3 Purified			FC	RUO

References

References: Tanaka Y, Koyanagi Y, Tanaka R, Kumazawa Y, Nishimura T, Yamamoto N. Productive and lytic infection of human CD4+ type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1. J Virol. 1997 Jan;71(1):465-70.

Hutchcroft JE, Tsai B, Bierer BE. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J Biol Chem. 1996 Jun 7;271(23):13362-70.

Adair JR, Athwal DS, Bodmer MW, Bright SM, Collins AM, Pulito VL, Rao PE, Reedman R, Rothermel AL, Xu D, et al. Humanization of the murine anti-human CD3 monoclonal antibody OKT3. Hum Antibodies Hybridomas. 1994;5(1-2):41-7.

Kung PC and Goldstein G. Complement-fixing monoclonal antibody to human T cells, and methods of preparing same. U.S. Pat. 4,361,549 dated Nov. 30, 1982.

Hoffman RA, Kung PC, Hansen WP, Goldstein G. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc Natl Acad Sci U S A. 1980 Aug;77(8):4914-7.

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Antigens	Area of Biology	Cell Type	Formats
CD3	Adaptive Immunity Cell Signaling	NK & NKT Cells T Cells Th17 Cells Treg Cells	Alexa Fluor 488