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Anti-Human CD278 (ICOS) Super Bright 436

Clone: ISA-3

RUO: For Research Use Only. Not for use in diagnostic procedures.

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SKU# 62-9948

Cat. No. Size
62-9948-41 25 tests
62-9948-42 100 tests

Data for Anti-Human CD278 (ICOS) Super Bright 436.

Staining of unstimulated (left) or Anti-Human CD3 and CD28 Functional Grade Purified (cat. 16-0037...View More

  • Data for Anti-Human CD278 (ICOS) Super Bright 436.

Description: The ISA-3 monoclonal antibody reacts with human CD278 also known as Inducible Costimulatory molecule (ICOS), H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (cat. 14-9949), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948 or 12-9948 rather than 11-9948.

Host Mouse
Isotype IgG1, kappa
Reactivity Human
Conjugate Super Bright 436
Laser Violet Laser
Emit 436 nm
Excite 405 nm
Legal Patent pending.
Reported Applications Flow Cytometric Analysis
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
14-9948 Anti-Human CD278 (ICOS) Purified FC, IHC, IHC-F, FA, IP RUO
61-9948 Anti-Human CD278 (ICOS) PE-eFluor® 610 488 - 561 nm 607 nm FC RUO
11-9948 Anti-Human CD278 (ICOS) FITC 488 nm 520 nm FC RUO
12-9948 Anti-Human CD278 (ICOS) PE 488 - 561 nm FC RUO
16-9948 Anti-Human CD278 (ICOS) Functional Grade Purified FC, FA RUO
25-9948 Anti-Human CD278 (ICOS) PE-Cyanine7 488 - 561 nm 775 nm FC RUO
48-9948 Anti-Human CD278 (ICOS) eFluor® 450 405 nm 445 nm FC RUO
47-9948 Anti-Human CD278 (ICOS) APC-eFluor® 780 633 - 647 nm 780 nm FC RUO
17-9948 Anti-Human CD278 (ICOS) APC 633 - 647 nm 660 nm FC RUO
13-9948 Anti-Human CD278 (ICOS) Biotin FC RUO
46-9948 Anti-Human CD278 (ICOS) PerCP-eFluor® 710 488 nm 710 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
00-6993 7-AAD Viability Staining Solution FC GPR
16-0289 Anti-Human CD28 Functional Grade Purified FC, FA RUO
SB-4400 Super Bright Staining Buffer FC GPR
16-0037 Anti-Human CD3 Functional Grade Purified FC, FA RUO
62-4714 Mouse IgG1 K Isotype Control Super Bright 436 405 nm 436 nm FC RUO

References: Quiroga MF, Pasquinelli V, Martínez GJ, Jurado JO, Zorrilla LC, Musella RM, Abbate E, Sieling PA, García VE.Inducible costimulator: a modulator of IFN-gamma production in human tuberculosis. J Immunol. 2006 May 15;176(10):5965-74.(ISA-3, FA, PubMed)

Grimbacher B, Hutloff A, Schlesier M, Glocker E, Warnatz K, Drager R, Eibel H, Fischer B, Schaffer AA, Mages HW, Kroczek RA, Peter HH. 2003. Homozygous loss of ICOS is associated with adult-onset common variable immunodeficiency. Nat Immunol. 4(3):261-8.

Buonfiglio D, Bragardo M, Redoglia V, Vaschetto R, Bottarel F, Bonissoni S, Bensi T, Mezzatesta C, Janeway Jr CA, Dianzani U. 2000. The T cell activation molecule H4 and the CD28-like molecule ICOS are identical. Eur J Immunol. 30(12):3463-7.

Beier KC, Hutloff A, Dittrich AM, Heuck C, Rauch A, Buchner K, Ludewig B, Ochs HD, Mages HW, Kroczek RA. 2000. Induction, binding specificity and function of human ICOS. Eur. J. Immunol. 30, 3707.

Hutloff A, Dittrich AM, Beier KC, Eljaschewitsch B, Kraft R, Anagnostopoulos I, Kroczek RA. 1999. ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28. Nature. 397(6716):263-6.

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