Anti-Human CD274 (B7-H1) PE-Cyanine7

Also known as: PD-L1

Clone: MIH1

RUO: For Research Use Only. Not for use in diagnostic procedures.

SKU# 25-5983

Cat. No. Size
25-5983-41 25 tests
25-5983-42 100 tests
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Data for Anti-Human CD274 (B7-H1) PE-Cy7.

Staining of unstimulated (left) or PHA-stimulated (right) normal human peripheral blood cells with ...View More

  • Data for Anti-Human CD274 (B7-H1) PE-Cy7.
Description

Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody.

Details
Host Mouse
Isotype IgG1, kappa
Reactivity Human
Conjugate PE-Cyanine7
Laser Blue Laser, Green Laser, Yellow-Green Laser
Emit 775 nm
Excite 488 - 561 nm
Legal FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APPLICATIONS. OTHER USE NEEDS LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. UNDER U.S. PATENT # 5,268,486, 5,569,587 AND 5,627,027 AND FOREIGN EQUIVALENTS AND PENDING APPLICATIONS. THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF GE HEALTHCARE BIO-SCIENCES CORP. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM GE HEALTHCARE BIO-SCIENCES CORP. THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH. IT IS NOT LICENSED FOR ANY OTHER USE. THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE. COMMERCIAL USE shall include: 1. sale, lease, license or other transfer of the material or any material derived or produced from it; 2. sale, lease, license or other grant of rights to use this Material or any material derived or produced from it; 3. use of this material to perform services for a fee for third parties. IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO EBIOSCIENCE, INC. 10255 SCIENCE CENTER DRIVE, SAN DIEGO, CALIFORNIA 92121 USA AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.
Reported Applications Flow Cytometric Analysis
Documentation
TDS Link Download TDS
Additional Formats
Cat. No. Name Excite Emit Application Reg.
16-5983 Anti-Human CD274 (B7-H1) Functional Grade Purified FC, FA RUO
17-5983 Anti-Human CD274 (B7-H1) APC 633 - 647 nm 660 nm FC RUO
46-5983 Anti-Human CD274 (B7-H1) PerCP-eFluor® 710 488 nm 710 nm FC RUO
13-5983 Anti-Human CD274 (B7-H1) Biotin FC RUO
14-5983 Anti-Human CD274 (B7-H1) Purified FC, IHC, WB RUO
12-5983 Anti-Human CD274 (B7-H1) PE 488 - 561 nm FC RUO
Related Products
Cat. No. Name Excite Emit Application Reg.
25-4714 Mouse IgG1 K Isotype Control PE-Cyanine7 488 - 561 nm 775 nm FC RUO
65-0865 Fixable Viability Dye eFluor® 780 FC GPR
References

References: Chen Y, Zhang J, Li J, Zou L, Zhao T, Tang Y, Wu Y. Expression of B7-H1 in inflammatory renal tubular epithelial cells.Nephron Exp Nephrol. 2006; 102(3-4):e81-92. (MIH1, IH/F, FA, PubMed)

Saudemont, A., Jouy, N., Hetuin, D., and Quesnel, B. 2005. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood. 105:2428-2435.

Ohigashi Y, Sho M, Yamada Y, Tsurui Y, Hamada K, Ikeda N, Mizuno T, Yoriki R, Kashizuka H, Yane K, Tsushima F, Otsuki N, Yagita H, Azuma M, Nakajima Y. Clinical significance of programmed death-1 ligand-1 and programmed death-1 ligand-2 expression in human esophageal cancer. Clin Cancer Res. 2005 Apr 15;11(8):2947-53. (MIH1, IH/F, PubMed)

Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. 2003. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 171(8):4156-63.

Freeman, G. J., A. J. Long, et al. 2000. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med 192(7): 1027-34.

Dong, H., G. Zhu, et al. 1999. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med 5(12): 1365-9.